Microbiology Practical - Word Count 0 Page 1 of 9 Word Count Page 01 of 9 An investigation into how the distribution of different leukocytes and their

Microbiology Practical - Word Count 0 Page 1 of 9 Word...

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The number of leukocytes differ in each individual. Blood assays are often the first diagnostic tool to allow insight into the cause of a patient’s symptoms. Data collected from 311 individuals was compared alongside published data. The aim of this practical was to learn how to correctly set up a light microscope to achieve optimal image resolution while understanding the limitations faced in viewing a specimen at various magnifications. Another aim was to prepare and examine a blood smear, distinguishing between the types of leukocytes with Leishman’s stain [1] . The experiment begun by retrieving a blood sample then producing a smear. A spring-loaded sterile lancet was used to puncture the thumb following sterilisation (aseptic technique). The blood was dropped onto a slide and another slide was used to spread the blood, ensuring the slide was pulled not pushed, as it would damage the erythrocytes. Leishman’s stain was dropped onto the smear, followed by a 6.8pH buffer solution in equal quantities. After 7 minutes, the slide was rinsed with distilled water and dried by tapping it onto filter paper, and then air dried [1] . An investigation into how the distribution of different leukocytes and their proportions vary in the blood in over 300 individuals using light microscopy. Fatema Mustansir Dawoodbhoy 0105859 Aims and Objectives Methodology Figure 1 Production of a blood smear on a slide without damaging the red blood cells [2] Word Count 0 Page 1 of 9 Word Count 0 Page 1 of 9
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To precisely focus a microscope, the main focusing knob (D) was used to focus the image on the lowest magnification. Next, the field iris (E) was closed and the condenser focus knob (F) was adjusted until a clear border was observed around the image. Then, the field iris (E) was opened gradually until the entire field of vision was just illuminated. The condenser iris (H) was fully opened and slowly closed, stopping when the image began to darken [1] . The leukocytes were counted systematically in clusters of 30-50 cells, identifying each type and calculating their percentages. Figure 2 Labelled diagram of a light microscope which was used in the practical [1] Word Count 0 Page 2 of 9 Word Count 0 Page 2 of 9
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Leukocytes are divided into two classes, granulocytes and agranulocytes [4] . Granulocytes have free visible granules and include basophils, neutrophils and eosinophils. Agranulocytes
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