Bio lab 1.pdf - Name Sarah Denison Section 553 Lab 1...

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Name: Sarah Denison Section: 553 Lab 1: Measurement, Pipette Use, and the Standard Curve Experiment 2 standard curve Table 1 Tube Final Protein Concentration µL of BSA protein stock (0.5 mg/ml) needed Bradford Reagent 0.15M NaCl for 2.0 mL final volume 8 50 µg/mL 200 µL 1.8 mL 0 µL 7 25µg/mL 100 µL 1.8 mL 100 µL 6 15 µg/mL 60 µL 1.8 mL 140 µL 5 10 µg/mL 40 µL 1.8 mL 160 µL 4 7.5 µg/mL 30 µL 1.8 mL 170 µL 3 5.0 µg/mL 20 µL 1.8 mL 180 µL 2 2.5 µg/mL 10 µL 1.8 mL 190 µL 1 0 µg/mL 0 µL 1.8 mL 200 µL Table 2 Final Concentration Absorbance 595 nm 50 µg/mL 1.022 A 25 µg/mL 0.613 A 15 µg/mL 0.548 A 10 µg/mL 0.378 A 7.5 µg/mL 0.186 A 5.0 µg/mL 0.260 A 2.5 µg/mL 0.154 A 0 µg/mL 0.000 A R 2 Part B: Protein Concentration in Milk Table 3 My milk type: 2% The dilution of milk I used was: 1:10
Tube Unknown Volume Bradford Reagent 0.15M NaCl for 2.0 mL final A 595nm Concentration measured by Spec. Concentration measured by Spec. 1 5 µL 1.8 mL 195µL 0.128 A 7.111 µg/ml 2.844 µg/µl 2 10 µL 1.8 mL 190 µL 0.401 A 22.278 µg/ml 4.456 µg/µl 3 20 µL 1.8 mL 180 µL 0.683 A 37.944 µg/ml 3.794 µg/µl 4 40 µL 1.8 mL 160 µL 1.066 A 59.222 µg/ml 2.961 µg/µl

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