BIO 201 Research Module #1 - Week 2.pdf - Week 2 – Analysis of unknown solutions and performing a literature search During this portion of the lab

BIO 201 Research Module #1 - Week 2.pdf - Week 2 –...

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Week 2 – Analysis of unknown solutions and performing a literature search During this portion of the lab, students will utilize scientific equipment and laboratory skills learned in previous labs to explore the chemical properties of various unknown solutions. Students will then analyze their results and utilize inductive and deduction reasoning skills to identify of each of the unknown solutions. Finally, students will identify questions that remain unanswered or gaps in their understanding of each unknown solution and perform a search of the scientific literature to answer their questions and fill in the gaps. For the second and third weeks of this research module, we will practice applying principles of the scientific method, along with techniques learned in the Skills Workshop #1 and Week #1 of this research module (solutions, pH, spectrophotometry) to evaluate the following two suppositions: 1) The ability to identify the stains depends on blood, wine, grape juice, and Kool-Aid having distinct chemical compositions that can be analyzed, compared, and then used as identifying features (chemical “fingerprints”). 2) Kosher wine, unlike non-kosher varieties, contains no protein (due to religious restrictions associated with the wine-making process). This must be taken into consideration when comparing chemical finger prints because the defendant (Ms. Higgins) says she does not remember if they purchased kosher or non-kosher wines. Something to think about: Can we also use a protein assay to determine if the stain is, or is not, Kool Aid? Each lab group will be provided a series of five samples – Tropical Fruit Kool-Aid, Concord grape juice, red wine (from concord grapes), kosher red wine (from concord grapes), and animal blood, but you will not be told which is which. You will be collecting a good deal of data – it is crucial that you record all the data in an organized and clear manner in your lab notebook. Discuss with your lab partners which data you will collect and how you want to set up any tables before you start collecting data. Also, be sure to include in your notebook any technical notes, questions, ideas, discussion or anything else that you think is important about the lab and that you may need/want to refer to in the future. Goggle and glove are REQUIRED for this portion of the lab! 1. Centrifugation is a quick and easy way to characterize solutions based on the presents (or the absence) of precipitates. For this analysis, you do not need to craft a null or an alternative hypothesis.
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