13 - BIO 305 Problem set for lecture 13 Winter 2008 1...

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BIO 305 Problem set for lecture 13 Winter 2008. 1. Concept question (B/C level). In a single sentence, describe the conceptual relationship between the following pairs of words. Be as precise as possible. Please note that there is not a single best answer for these questions. As long as your answer demonstrates that you are understand the concepts, it will be marked as correct. Pairs of concepts: (a) splicing – protein (b) polycistronic – ribosome (c) mRNA – snRNA (d) aminoacyl-tRNA synthetase – anticodon (e) degenerate genetic code – wobble Ans: Examples include: (a) Prior to protein synthesis in eukaryotes, the splicing machinery remove introns from primary RNA transcripts to generate mRNA. (b) On polycistronic mRNAs, the ribosome can enter at multiple sites to initiate protein synthesis. (c) snRNAs are components of the spliceosome which is part of the cellular machinery that processes primary RNA transcripts into mRNAs. (d) Aminoacyl-tRNA synthetase enzyme specifically recognize both tRNA molecules with specific anticodons as well as amino acids. (Note they don’t just recognize one anticodon. They recognize all tRNAs with anticodons for a single amino acid). (e) In some cases, the multiple codons coding for a single amino acid in the degenerate genetic code can be bound by the same tRNA using wobble interactions. 2. (B level) You are examining mutant forms of a new protein and find that most often the mutations affect a single amino acid (#69), as shown below.
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