Ch 15 lectures.ppt - Gene expression an epic journey • Transcription • RNA processing • Translation The Genetic Code Translation Chapter 15 15.1

Ch 15 lectures.ppt - Gene expression an epic journey •...

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Gene expression: an epic journey Transcription RNA processing Translation
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The Genetic Code & Translation Chapter 15 15.1 – One gene, one enzyme hypothesis Structure & function of proteins 15.2 – Genetic code 15.3 – Translation 15.4 – 3D structure of the ribosome – Polyribosomes (skip rest of this section, at least for now)
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Where biochemists ruled, genetics was considered a second-class pursuit…. But the following experiments were about to change everything….. In a world….
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Neruosopra (bread mold) Can grow in rich media (all amino acids supplied) or “minimal media” no amino acids
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Isolation of auxotrophic (unable to produce a particular nutrient) mutants Beadle & Tatum
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Genetic dissection of biosynthetic pathway Srb & Horowitz Arginine synthesis
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Genetic dissection of biosynthetic pathway TABLE 15.1 Growth of arginine auxotrophic mutants on minimal medium with various supplements Mutant Strain Ornithine Citrulline Arginine Group I + + + Group II + + Group III + Note: A plus sign (+) indicates growth; a minus sign (−) indicates no growth.
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Genetic dissection of biosynthetic pathway Srb & Horowitz Arginine synthesis One gene codes for one enzyme
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Concept Check Auxotrophic mutation 103 grows on minimal medium supplemented with A, B, or C. Mutation 106 grows on medium supplemented with A and C, but not B; and mutation 102 grows only on medium supplemented with C. What is the order of A, B, C in a biochemical pathway?
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Concept Check Auxotrophic mutation 103 grows on minimal medium supplemented with A, B, or C. Mutation 106 grows on medium supplemented with A and C, but not B; and mutation 102 grows only on medium supplemented with C. What is the order of A, B, C in a biochemical pathway?
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