yeastlab

yeastlab - Christopher Nolop 18 February 2008 Biology 14...

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Christopher Nolop 18 February 2008 Biology 14 Lab #1 Testing for When Adaptive Copper Resistant Mutants Occur in Baker’s Yeast Colonies ( Saccharomyces cerevisiae) on Agar Media Hypothesis: I believe that the majority (>50%) of the yeast colonies on the two copper medium plates will arise in the same location, indicating that these copper resistant mutants arose before their exposure to the copper agar and not after . Methods: Agar was chosen as the media for the growth of the yeast colonies for its sterility, its stability over a wide range of temperatures, and its non-degradation by microbes . Yeast was chosen as the subject organism because of its rapid reproductive cycle and for the simplicity of analyzing its results . A sterilized agar medium without copper in a sterilized flask was poured (roughly 20 ml) into a Petri dish . To obtain the right amount of yeast cells, a 0 . 01 % suspension of physiological saline solution and yeast was concocted . A single drop of the resulting solution was spread onto the agar medium with a sterilized spreader . The sealed original yeast culture was left to cultivate upside down (to prevent condensation from dripping onto the culture) at room temperature for 2-3 days . A new copper containing agar medium was poured into two newly labeled Petri dishes (“+Cu1” and “+Cu2”) with vertical marks
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This note was uploaded on 04/01/2008 for the course BIO 14 taught by Professor Chew during the Spring '08 term at Tufts.

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yeastlab - Christopher Nolop 18 February 2008 Biology 14...

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