DNA_synthesis - LEARNING OBJECTIVES Draw and describe the...

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LEARNING OBJECTIVES Draw and describe the molecular mechanisms that result in DNA synthesis. In your description you should include the following terms: Origin of replication single strand binding protein replication fork sliding clamp leading strand DNA polymerase I lagging strand DNA polymerase III Okazaki fragments RNA primers Primase helicase Topoisomerae ligase Understand the role of RNA primers in DNA replication. Explain why continuous DNA synthesis is not possible on both DNA strands in both directions. Distinguish between the leading strand and the lagging strand replication. Comprehensive Model for DNA Synthesis (Table 14.1) A. Observations of How DNA Synthesis Occurs in Vivo 1. DNA polymerases add dNTPs only to the 3’ end of an existing strand. ( Fig. 14.10 ) 2. DNA synthesis always proceeds in the 5’ to 3’ direction. 3. DNA polymerase can add only to an existing 3’-OH group; therefore, it needs a short oligonucleotide primer to begin replication.
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  • Spring '07
  • Luckie
  • DNA

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