Chapter 6 Study Guide.pdf - Chapter 6 Study Guide To be prepared for the in class quiz and assignments please answer the following questions by reading

Chapter 6 Study Guide.pdf - Chapter 6 Study Guide To be...

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Chapter 6 Study Guide To be prepared for the in class quiz and assignments, please answer the following questions by reading the text or listening to lecture videos. Section 6.2 How do you make a pure culture of bacteria? isolation/dilution streaking -liquid culture on an inoculating loop and streaked across creating individual colonies of bacteria on an agar plate. Organisms fall off the loop as it moves along the agar surface. Towards the end, few bacteria remain on the loop Spread plate technique - tenfold dilutions (transferring 1ml of a broth w bacteria to a 9ml sterile broth. Early dilutions contain more bacteria producing a confluent (lawn on organism covering a surface) growth What is the definition of the following terms: Complex media - aka rich medium, nutrient rich growth solution including undefined chemical components such as beef broth ex. Poorly defined ingredients; yeast or beef extract E.coli or bacillus subtilis Enriched media - growth solution for fastidious bacteria, consisting of complex medium plus additional components Ex. Amino acids, peptides, vitamins, sugars, or blood H. influenzae or neisseria meningitidis Selective media - medium that allows the growth of certain species or strains of organisms but not others ex. Salmonella enterica Differential media - growth medium that can distinguish between various bacteria on the basis of metabolic differences. Exposes biochemical differences between the two species that grow equally well How do we accomplish the following methods of counting bacteria: Microorganisms can be counted directly using a microscope Direct counting - using the Petroff-Hausser Chamber ; number of organisms counted within that volume is used to calculate the concentration of cells in the original culture ex. Figure 6.9 page 166
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Seeing an organism doesn’t mean that it is alive. Only dead cells stain red under fluorescence scope… orange or yellow Living cells look green Fluorescence-activated cell sorte r (FACS) - count cells and sort them according to protein differences in fluorescence direct Viable counts - dilutions of a liquid culture can be plated directly on an agar surface. After they form, they are counted, and the original cell number is calculated
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