Chapter4_16x9.ppt - Chapter 4 Nucleic Acids 1 2 3 4 5 6 7...

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Chapter 4: Nucleic Acids
1.Serve as information carriers2.Basis for energy storage (ATP)3.Basis for enzymatic co-factors4.Basis for signaling molecules5.Mediate protein synthesis6.Pre-biotic RNA world?7.Used in research and medicine
COMMON THEMESThe SAME forces will be at work all year in BBMB 404:HYDROPHOBIC EFFECTH-BONDINGELECTROSTATIC INTERACTIONSVAN DER WAALS…along with COVALENT BONDS
Nucleotide structure and nomenclature
Sugars in nucleic acids
Glycosidic LinkageA nucleoside1’5’ esterification5’9’RNA: adenosine, guanosine, cytidine, uridineDNA: deoxyadenosine, deoxyguanosine, deoxycytidine, thymidine
A nucleotide: ATP5’ ATPWhat would 3’ dGMP look like?
Watson-Crick model of ds-(B form) DNA1. Antiparallel coil around a common axis2. Sugar-phosphate on the outside, bases on the inside3. Bases are perpendicular to the sugar-phosphate axis4. Translation is 3.4 Å per base, repeats every 10 bases (or 34 Å).
DNA Anti-parallel strandsSugar-phosphate backbone, H-bonded basesMajor groove vs. minor groove10 bases/turnDiameter is 20ÅStabilizing forces
5’3’5’3’5’3’The DNA double helix is antiparallel (and thus each strand has direction)Phosphodiester bond(across the sugar)
DNAKnow this structure!Phosphodiester linkage
DNA Anti-parallel strandsSugar-phosphate backbone, H-bonded basesMajor groove vs. minor groove10 bases/turnDiameter is 20ÅStabilizing forces
6’1’3’4’6’1’2’3’4’2’
DNA Anti-parallel strandsSugar-phosphate backbone, h-bonded basesMajor groove vs. minor groove10 bases/turnDiameter is 20ÅStabilizing forces
The reason for different grooves….
DNA Anti-parallel strandsSugar-phosphate backbone, h-bonded basesMajor groove vs. minor groove10 bases/turnDiameter is 20ÅStabilizing forces

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