Lab 6 - Crayfish Physiology

Lab 6 - Crayfish Physiology - Anil Kanungo BIO 206L...

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5/7/2009 Anil Kanungo BIO 206L Thursday 6-10pm Lab #6: Crayfish Physiology Lab Crayfish: Time (min) Oxygen Level Oxygen Conc. Water Pumped (#mL/ 4 min) Water Pumped (#mL/ min) Total Water Pumped (mL) 0 High 7.2 0 0 0 4 7.38 21 5.25 21 8 7.1 25 6.25 46 12 7.12 22 5.5 68 16 7.07 20 5 88 20 7.01 19 4.75 107 24 Low 5.31 30 7.5 30 28 4.37 34 8.5 64 32 3.81 35 8.75 99 36 3.83 36 9 135 40 High 6.41 24 6 131 44 7.26 17 4.25 148 48 6.55 27 6.75 175 52 7.37 28 7 203 56 7.2 28 7 231 60 7.15 26 6.5 257 This graph compares how the water pumped by the crayfish varied with oxygen concentration. It is evident that as oxygen decreased, the crayfish pumped more water to try and compensate for the lack of O 2 . The table shows the graphed values including the water pumped per 4 minutes (which are plotted) and also the calculated water pumped per minute. The oxygen level was set to high, then low, then back to high. Study Questions: 1.) Make a graph of the results you obtained in this exercise. Indicate the start time as time (zero) on the horizontal axis, and then mark off 5-minute time intervals for the duration of the exercise. On the lower portion of the vertical axis, mark off oxygen levels in mg/L throughout the range at which they were measured. On the upper portion of the vertical axis, mark off ventilation rate in # mL / min throughout the range at which rates were measured. Plot the data for ventilation rate and oxygen Page 1 of 6
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5/7/2009 Anil Kanungo BIO 206L Thursday 6-10pm concentration, connecting the data points for each with straight lines. Graph shown above. Note: instructions were to connect the plotted points with straight lines but the example presented in the manual showed smoothed lines. I felt smoothed lines would give a more accurate representation. 2.) Discuss the results you obtained in this exercise in terms of the crayfish’s physiological responses to the different treatments. Did you observe any differences between the initial response to a change in oxygen concentration and the subsequent response to continued exposure to the same oxygen concentration? Was the crayfish’s ventilation rate during the second exposure to a high oxygen concentration the same as the rate before the exposure to a low oxygen concentration? Suggest an explanation for the results you obtained. The crayfish’s ventilation rate was generally constant but there were some spikes. The transitions between the oxygen concentrations showed the crayfish to be ventilating faster than needed. After some time, the crayfish would apparently calm down and ventilation would approach a more constant and steady rate. The higher ventilation rates at
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