Lab 7 - Animal Development & Fungal Reproduction

Lab 7 - Animal Development & Fungal Reproduction - Anil...

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5/11/2009 Anil Kanungo BIO 206L Thursday 6-10pm Lab #7: Animal Reproduction and Development/Fungal Reproduction Lab ANIMAL REPRODUCTION AND DEVELOPMENT: Summary: Exercise B: Observations of sea urchin fertilization and early cleavage: A video was shown demonstrating how living eggs and sperm were obtained from sea urchins. Students were then furnished with “dry” sperm and a vial of eggs. Students then took a depression slide and placed a drop of eggs into the depression. Next, using a toothpick, students took sperm and swished it around the depression slide containing egg. A coverslip was applied to the slide and the specimen was viewed under high magnification. Exercise D: Observations of hyper- and hypo-tonic solutions on sea urchin eggs Students used various solutions to examine the effects of hyper- and hypo-tonic solutions on sea urchin eggs. Students began by placing a few urchin eggs with sea water in a depression slide. They observed this under a microscope. Next, students placed hypertonic saline on one side of the coverslip and drew the solution into the depression with a Kimwipe. The urchin eggs were observed to shrivel up and die when coming into contact with this solution. Students then repeated the process with the other side of the coverslip and a hypotonic solution. This time the urchin eggs were observed to swell and eventually burst. Exercise E: Observations of whole chick embryos and embryo sections at 33, 72, and 96 hours of incubation The objective of this exercise was to obtain a chick embryo from an egg that had been incubated for 33, 72, and 96 hours. Our table was unable to successfully obtain an embryo from these eggs so we were forced to use the prepared examples. At 33 hours, the embryo resembled a very large sperm cell. It was worm-like with signs of the brain regions developing. The heart was also very obvious on the right side of the embryo. The notochord could be seen as a solid rod extending to the brain. By 72 hours, the embryo had made major growth progress and the anterior end had begun to curl down towards the body of the embryo. The central nervous system and sense organs had advanced drastically. The heart had also twisted to form its chambers. By 96 hours, the embryo had enlarged and curled more. Organs are now visible.
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