dragon fruit final.pdf - IJBPAS June 2017 6(6 1169-1180...

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IJBPAS, June, 2017, 6(6): 1169-1180 ISSN: 2277–4998 1169 IJBPAS, June, 2017, 6(6) PHYTOCHEMICAL SCREENING AND BIOASSAY OF THE ANTIBACTERIAL ACTIVITY OF Hylocereus undatus and Hylocereus polyrhizus FRUIT PEEL ROMERO, JOANA G 1 , WAING, KRISTINE GRACE D 1 , AND VALENTINO, MARY JHANE G 1* 1 Department of Biological Sciences, College of Arts and Sciences, Central Luzon State University, Science City of Munoz, Nueva Ecija, 3120 Philippines *Corresponding author: [email protected] ABSTRACT This study was conducted to screen the presence of tannins, steroids, saponins, flavonoids, alkaloids, terpenoids, and cardiac glycosides in ethanol and hot water extracts of Hylocerus undatus and Hylocerus polyrhizus fruit peels. Antibacterial properties of the aforementioned dragon fruit peels as protectant and eradicant against Escherichia coli 25922 and Staphylococcus aureus 25923 were also evaluated. Phytochemical screening showed that H. undatus and H. polyrhizus fruit peel extracts contains terpenoids and cardiac glycosides. While steroids and saponins are present only in H. undatus and H. polyrhizus ethanol extracts. Antibacterial activity as eradicant of ethanol extract of H. undatus and H. polyrhizus fruit peel and protectant activity of H. undatus ethanol extract, H. polyrhizus fruit peel hot water and ethanol extracts against E.coli at 12, 24 and 36 hrs of incubation were observed. Keywords: antibacterial, dragon fruit, eradicant, phytochemical, protectant Received 29 th December 2016; Revised 20 th January 2017; Accepted 26 th March 2017; Available online 1 st June
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Romero JG et al Research Article 1170 IJBPAS, June, 2017, 6(6) INTRODUCTION Dragon fruit or Pitaya was first introduced in the Philippines in 1990s in Ilocos region where it was locally called as Sanaita [1]. Dragon fruit is a perennial, epiphytic, climbing cactus with a triangular fleshy jointed stems which belongs to family Cactacea and of genus Hylocerous [2, 3]. There are three varieties of dragon fruit which include Selenicereus megalathus or white flesh with yellow peel dragon fruit, Hylocereus undatus or white-flesh with red peel dragon fruit and Hylocereus polyrhizus or red-flesh with red peel dragon fruit. They are rich in vitamins, fiber, phosphorus, calcium, magnesium, calcium, magnesium, phytochemicals and antioxidants [4]. Accordingly, Yong [5] revealed that dragon fruit is also known to possess medicinal properties that could prevent cancer, diabetes and neutralizes toxins in the body. It is also helpful in reducing blood sugar levels in people suffering from type 2 diabetics [6, 7, 8]. In the past decade interest on the topic of antibacterial property of plant extracts which can be attributed to their bioactive compounds has been growing and researchers attributed this to the presence of several bioactive compounds [9].
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