Notes - Chapter 7 A Cell(Not a Plant The areas of the cell...

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Chapter 7A Cell (Not a Plant)The areas of the cell not with photobleaching light would have migrated if the membrane is fluid.If the membrane is fluid, the expectation (area treated) will become green again In this case think about it in a lateral diffusion NOT movement down the concentration gradient The treated area received the treatment You have 100% of proteins in the bleached area able to emit colorLateral diffusion is randomPhotobleaching = removing lightFlorescent Lostin Photobleaching Experiment Instead of having a finite amount of time that we are radiating laser, we will turn that laser on until the experiment is completeThere are 2 discrete regions of cell: bleached area and measured area (the area not interacting with the laser beam) Laser stays onTwo areas are observed The membrane protein is not being denatured. Denaturation we are turning it back to the primary level of structure.Cell becomes void in color eventually (lacking green fluorescence); new proteins moving in, being bleached, moving outThere is a gradual loss of flouresenseBoth experiments support membrane fluidity but in different waysHow are both experiments similar/ different/ how do both support membrane fluidity?3 major factors that affect membrane fluidityLengthDouble bondsPresence or absence of cholesterolLonger length (tails) of bonds means more opportunities for hydrophobic interactions. The more hydrophobic interactions that take place, the less fluid the membrane becomes.

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