WhatsApp Image 2017-12-11 at 6.31.02 PM.jpeg - the immobilized ligand whereas other of substances are washed through the in column with the buffer SDS

WhatsApp Image 2017-12-11 at 6.31.02 PM.jpeg - the...

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Unformatted text preview: the immobilized ligand, whereas other of substances are washed through the in column with the buffer . - SDS . PAGE separates proteins by mass An 8DS- PAGE, the diligent SDS to added to denature proteins . Proteins assume a nod like shape in the presence of Sos . SDS interfere with the hydrophobic interactions that normally stabilise proteins. The net result is that I SDS - treated proteins have similar shapes and charge- to -runso Ration . - gsoelectric to cussing If a mixture of protems is electrophoresis through a sol" or gel that has a stable PH gradient Smoothly T Les in which the pit from anode to cathode , each protein will migrate to the position in the ph gradient corresponding to its PI. If a protem molecule diffuses away from the position , its net charge will change as it moves into a region of different ph and the resulting electrophoratic forces will move it back to it's isoelectric position. Each specie...
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