Unformatted text preview: the immobilized ligand, whereas other
substances are washed through the
column with the buffer .
- SDS . PAGE separates proteins by mass
An 8DS- PAGE, the diligent SDS to added
denature proteins . Proteins assume
a nod like shape in the presence of
Sos . SDS interfere with the hydrophobic
interactions that normally stabilise proteins.
The net result is that I SDS - treated
proteins have similar shapes and
charge- to -runso Ration .
- gsoelectric to cussing
If a mixture of protems is electrophoresis
through a sol" or gel that has a
stable PH gradient
Smoothly T Les
in which the pit
from anode to cathode ,
each protein will migrate to the
position in the ph gradient corresponding
to its PI. If a protem molecule diffuses
away from the position , its net charge
will change as it moves into a region
of different ph and the resulting
electrophoratic forces will move it back
to it's isoelectric position. Each specie...
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- Summer '17