Fluorescence Microscopy Question SP2018 Copy.docx - Name Biology 351 SP2019 Fluorescence Microscopy Questions – Quiz 3(25 pts Do your own writing in

Fluorescence Microscopy Question SP2018 Copy.docx - Name...

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Name: __________ ____ __________ Biology 351 SP2019 Fluorescence Microscopy Questions – Quiz 3 (25 pts). Do your own writing in answering questions, write clearly with good sentence structure, grammar and spelling. As a take home quiz, use any resource available to help answer these questions. However, d o not work with others on the quiz . Due 03/20 at 5 PM by email. 1. The integrin receptor provides special functions to cells. Describe how it works to stabilize cell adhesion to its base for long-term attachment. Also in this instance, give one example of intracellular signaling it performs for a specific purpose in the cell. 3 pts 2. The fluorescence microscope has a main purpose to separate light into two pathways that allows a fluorescent tag to be seen in a specimen. Name the filters in the cube that makes possible fluorescence microscopy? What is the specific function of each of the filters? 6 pts a. _____________ b. _____________ c. _____________ 3. A specimen is immunolabeled with “ rabbit anti-mouse Alexa 488
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Unformatted text preview: ”. Describe the name’s meaning as information for each part. 3 pts 4. You want to immunolabel a very low abundance expressed plasma membrane protein in cells. What would be your best approach to labeling this protein? Describe your approach to use with an upright fluorescence microscope. 3 pts 5. The green fluorescence protein was discovered in the A. victoria jellyfish. Report how the green fluorescence protein fluoresces in jellyfish. Describe the reaction details in your own words ! 3 pts 6. I 4 pts A. Fluorescein isothiocyanate dye B. Aequorin protein C. Tetramethylrhodamine dye D. Acridine Orange dye E. Alexa 345 dye F. Alexa 488 dye G. DAPI dye *Explain why your chosen two fluorophores work best together in combination to label the specimen. 8. Describe how colchicine worked to inhibit its target cytoskeleton. Colchicine is a useful inhibitor to stop mitosis activity, but why is it not a good choice for use in killing cancer cells in the body? 3 pts...
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