Visualizing the fluorescent mammalian expressing by using green fluorescence protein takes
many steps and important techniques before. Cell seeding first and count cell concentration.
Consequently, introducing the recombinant plasmids into mammalian cells that called
transfection. Transfection is the process of deliberately inserting naked or purified nucleic acids
Unformatted text preview: into eukaryotic cells. Transfection actually can be made by many methods as chemical, biological, or physical methods. Specially, chemical method is used which is lipid-based transfection method. Visualizing the EGFP protein after it’s expression in the mammalian cells it is the target finally of the previous experiments....
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- Fall '19
