17 lab ex1 sum07 key - Biol o gy 1 A La b Ex am#1 J uly 26...

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Summer 2007 Lab exam 1 Key Biology 1A - Lab Exam #1 – July 26 th , 2007 Answers Exam 1 Bio 1A, Summer 2007 1 B 6 C 11 A 16 C 21 D 26 B 2 E 7 E 12 E 17 C 22 B 27 E 3 D 8 B 13 E 18 A 23 B 28 D 4 B 9 A 14 E 19 B 24 C 29 B 5 A 10 D 15 C 20 E 25 D 30 Mean =60.75, STDEV = 13.3 . A (some form) 70-100, B (some form) 59-69, C (some form) 47-58, D (some form) 39- 46, F X < 39. Short answers are on the back. 2) From the curve you determine the original conc. Is 50 mM, too dilute to make a 100 mM unless boil away, etc. Units matter! 5) 1N = 2, 2N = 4. For mitosis 4 chromsomes with sister chromatids line up. No 1N cell in animals undergo mitosis. 6) In portion 3 additional enzyme does not change the O.D. which means all S converted to P in the time course of the experiment (substrate is limiting, enzymes are idle). 8) You don’t change the ploidy but instead change the amount of DNA, C value increases. 9) G2 = 4C and zygote = 2C. Determine #bp for 4.8 X 10 -11 grams. 4.8 X 10 -11 grams /6.0 X 10 2 g/mole bp = 0.8 X 10 -13 moles X 6.0 X 10 23 (Avogadros #) = 4.8 X 10 10 . 10) Note units, 400 cells/ul there need to do 1:4 dilution and then 2 serial dilutions of 1:10.
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