micro lab notes 1 .pdf - Tuesday September 3 2019 Lab Notes 1 Microbes bacteria grown in lab on cultured media • need carbon nitrogen minerals and

micro lab notes 1 .pdf - Tuesday September 3 2019 Lab Notes...

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Tuesday, September 3, 2019 Lab Notes 1 Microbes - bacteria grown in lab on cultured media need carbon, nitrogen, minerals and growth factors Most bacteria —> chemo-heterotrophs microbes are everywhere - Need to distinguish chemically defined medium —> know exactly what is in it, know exact chemical composition - Complex defined medium composition can vary slightly extract usually means it is complex medium - 1st bacterial cultures (liquid) bacteria grown in broth - Agar makes it solid but @ 100 C —> liquid but at 40 C it is solid - Boiling not e cient method of sterilisation - steam sterilisation —> autoclaving
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Unformatted text preview: • autoclave heats up to 121 C at 15 psi for 15 minutes -Grow on plate —> colonies (individual O growths) -turbid —> bacterial growth in liquid broth makes it appear cloudy -3 types of tubes —> broths, slants, deeps • broths—> large of growth in small space (liquid) • slants —> solid growth surface like a plate (solid) • Agar deeps—> grow bacterium and determine oxygen requirement (solid) • Semi solid deeps—> determines motility of species (solids and liquids) -loops inoculate —> broths, slants and plates 1 Tuesday, September 3, 2019-needles —> only for deeps 2...
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