Lab Test Study Guide - F19.pdf - Cell Biology Lab Test Study Guide The lab test will be held in the Potter Auditorium(Room 1028 in the Rowe Building on

Lab Test Study Guide - F19.pdf - Cell Biology Lab Test...

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Cell Biology Lab Test Study Guide The lab test will be held in the Potter Auditorium (Room 1028) in the Rowe Building on Tuesday, December 3 rd , from 9:35am – 10:25am. To help you prepare, I have included a list of words and some key concepts from the labs you should understand. (Not necessarily 100% complete). Lab 1: Cell Culture Identify: Ptk2 cells Trypsin Concepts: Background on Biosafety Understand various types of tissue culture equipment Procedure to subculture cells Lab 2: Early Apoptosis Identify: Trypan Blue Phosphatidylserine Annexin-V Propidium iodide Concepts: How to read a micropipettor How to set up a microscope for Kohler illumination Why do you use Phase Contrast, and how is it set up? How does the Annexin-V assay work and what does it tell you? How does fluorescent microscopy work? Lab 3: Electrophoresis Identify: SDS Mercaptoethanol (or DTT, they do the same thing) Glycerol Tracking dye Coomassie Blue Protein markers Sample Buffer Nitrocellulose
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