Enzyme Catalysis.docx - Enzyme Catalysis Pre-Lab Questions 1 How could you test to see if an enzyme was completely depleted during an experiment By

Enzyme Catalysis.docx - Enzyme Catalysis Pre-Lab Questions...

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Enzyme Catalysis Pre-Lab Questions 1. How could you test to see if an enzyme was completely depleted during an experiment? By increasing the amount of saturation, if the amount of reaction does not change then that means the enzyme was completely saturated. 2. Describe an experiment that could test the hypothesis that an enzyme binds to a substrate when it acts. An experiment to test an enzyme binding to the substrate to convert into a product could be: Starch -- amylase -- Maltose + Maltose. Amylase is an enzylme that exists in the saliva which catalyzes the hydrolysis of the polysaccharide starch to the disaccharide maltose. If amylase is added to starch the starch will be digested by the enzyme becoming maltose. One will disappear and the other will appear. 3. List three conditions that would alter the activity of an enzyme. Be specific with your explanation. The concentration, PH levels, and the temperatures. The amount of enzymes in a concentration compare to the substrate will effect how they react. The PH balance or acidity levels will affect their reaction. The temperature of the enzymes will affect if they denature or their reaction rate; Extreme hot or cold temperatures are not effective for enzymes. 4. Take a look around your house and identify household products that work by means of an enzyme. Name the products and indicate how you know they work with an enzyme. Some obvious household products that would work by the means of an enzyme would be washer or dishwasher detergents. Another would be common cleaners. If a protein has a biochemical reaction, then it is an enzyme. If the product contains a protein, then it will work with enzymes.
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Experiment 1: Enzymes in Food Data Tables Table 1: Substance vs. Starch Presence Substance Resulting Color Presence of Starch?
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