Lab 1 - Cell Culture.pdf - Lab 1 Mammalian Cell Culture Techniques(i.e how we grow cells in the lab(Photo M McCarville Assessments and preparation

Lab 1 - Cell Culture.pdf - Lab 1 Mammalian Cell Culture...

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1 Lab 1: Mammalian Cell Culture Techniques (i.e. how we grow cells in the lab) (Photo: M. McCarville) Assessments and preparation related to this lab: Watch the lab overview video on Brightspace. Complete the online pre-lab quiz before coming to lab (available 3 days before your lab session). Review the section in your textbook on apoptosis. Complete Study Proposal Part I (just one form per pair!) during the lab and submit it before you leave. Bring in a short Introduction when you come in for Lab 2. Welcome to Cell Biology! The labs for BIOL 2020 have been designed as a full term, multi-week apoptosis project that will take you through a complete workflow similar to what would happen in a real research lab. The material in Labs 1-4 will be compiled into a lab report that will be submitted in Lab 5. Today’s lab will be an introduction to the fundamental techniques of growing and maintaining mammalian cell lines in a lab. You will also be introduced to the workflow for the upcoming labs and will start planning the experiment that you will begin in Lab 2. Today: In order to provide more individual attention, you will be rotated through stations during the lab. J Part I (in the Cell lab!) Assemble in the cell lab (LSC 7012) for an introduction, including an overview of what to expect in the laboratory component, a description of the cell line that we will be using in the BIOL 2020 labs, and a lesson in sterile technique (basically, the MOST important aspect of mammalian cell culturing!!). Part II (in the Tissue culture room!) Two benches at a time will move to a small adjacent room to practice sterile technique before continuing to a tissue culture room. One bench (i.e. 4 students) will go to the tissue culture room on the 6 th floor, and the other bench will go to the tissue culture room on the 1 st floor. Here, we will walk you through the process of subculturing Ptk2 cells (a marsupial cell line). This will take about 30 minutes. Part III (in the Cell lab) In preparation for the upcoming labs, your TA will discuss your multi-week apoptosis project. You will complete the one page Study Proposal form (one form per pair) and hand it in today.
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2 Background on the Ptk2 Cell Line: This term BIOL 2020 students will use a marsupial cell line (Ptk2 cells) for all the experiments! These cells are epithelial kidney cells from a male potoroo, Potorous tridactylis . In 1962, Kirsten Walen and Spencer Brown isolated kidney cells from this small marsupial and put them in culture dishes, where they continued to replicate (Walen and Brown, 1962). Note that despite their rat-like appearance, and the common name ‘rat-kangaroos’, potoroos are indeed relatives of the kangaroo, and are not rodents! As you might expect, potoroos are found in the wild in Australia.
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  • Fall '11
  • Pohajdak

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