Lab 5 Intro Assignment.doc - Cell Biology Lab 5 Intro...

This preview shows page 1 out of 1 page.

Cell Biology Lab 5 Intro Assignment Katelyn Cisneros 901 Title (1pt): Bio-Engineering using Sequence Analysis and Western Blot testing to analyze Recombinant Dihydrofolate Reductase (DHFR) Questions/Purpose (2pt): For this experiment we are analyzing 3 DHFR proteins through the purification and detection of His-tagged, GST-tagged, and Myc-flag tagged proteins. First, we will analyze fusion proteins through sequence analysis and then use Western Blot. Through Western Blot we will analyze proteins experimentally to determine their molecular weights, the site of protein tag. (1) Hypothesis (1pt): The His-tagged DHFR protein is expressed and purified from E. coli. The yield of protein in this bacteria will be higher. Furthermore, the yield of His-tagged proteins will be the most and have the larger molecular weight. Rationale for your hypothesis (2pt): The yield of His-tagged protein will be higher because it is relatively easy to grow from E. coli bacteria. Also using bacteria as a host cell type for cell-

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture