partial lab report 1 cell bio.docx - Protein Quantification Katelyn Cisneros Kara Lamb Dusan Nikolic-Dorschel Aaron Parise Cell Biology PCB3023-901

partial lab report 1 cell bio.docx - Protein Quantification...

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Protein Quantification Katelyn Cisneros, Kara Lamb, Dusan Nikolic-Dorschel, Aaron Parise January 27, 2019 Cell Biology PCB3023-901
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Results: In our experiment we tested the absorbance at 595 nm due to the fact that the Coomassie Brilliant blue dye absorbs best in that range. We chose to test at intervals of 500 from concentrations of 100 to 2000 to get data to form our standard curve. Results can be seen in Table 1. Table 1: Absorbance of Samples taken with Spectrophotometer. The absorbance of tube 1 is 0.063, the absorbance of tube 2 was 0.221, the absorbance of tube 3 was 0.337, the absorbance of tube 4 is 0.479, the absorbance of tube 5 was 0.574. Table 2: Unknown Sample “C” and Absorbance The results for our unknown sample “C” were as follows, 1:1 was 1.07, 1:5 was 0.354, 1:10 was 0.232, and lastly 1:20 was .104. Absorbance (595 nm) Tube # Sample 0 0 0 0.063 1 100 0.221 2 500 0.337 3 1000 0.479 4 1500 0.574 5 2000 0 n/a 0 1:1 1.07 1:5 0.354 1:10 0.232 1:20 0.104
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From values we obtained from our test tubes and the absorbance we form a Standard curve graph. This curve gives us our y=mx+b equation and the R^2 value. The equation the curve
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