Molecular Investigation of Mutant Alleles.docx - MOLECULAR INVESTIGATION OF MUTANT ALLELES BY KATELYN CISNEROS GROUP 2 ROXANNE CHAVIANO DANIELA RIEUMONT

Molecular Investigation of Mutant Alleles.docx - MOLECULAR...

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M OLECULAR I NVESTIGATION OF M UTANT A LLELES B Y K ATELYN C ISNEROS G ROUP 2 ROXANNE C HAVIANO , D ANIELA R IEUMONT , M ALIK S ALAH -U D -D IN G ENERAL G ENETICS L AB PCB3063L-010
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Introduction Drosophila Melanogaster is a species of fly that is more commonly known as the fruit fly. The fruit fly isn’t just a pest around the kitchen, it’s much more than that. This organism has been used to define more fundamental genetic principles than any other multicellular eukaryote (CMMB, 2019). Drosophila is a perfect genetic model because their genome is sequenced, they are easy to maintain, manipulate and are cost effective. It usually takes one day for the eggs of the fruit fly to turn into larvae. Depending on the temperature, the progression of egg to adult usually takes about two weeks (CMMB, 2019). Drosophila can inherit mutations, such as wing type, eye color, and body color. The wildtype fruit fly is the most common phenotype found in nature. It has normal wings, brick red eyes, and a tan body. The epidermal and cuticle cells of the fly contain black melanin and appear as black stripes on the abdomen of the wildtype fruit fly. In order for the production of black melanin in these types of cells, they require yellow protein. So, if there is no yellow protein, the fly would exhibit a mutant yellow body color phenotype. The purpose of the experiment is to determine what DNA mutations are responsible for the mutant yellow body color phenotype. We will use the wildtype as a control group and the mutant yellow flies as the experimental group to determine the difference. We can estimate the genome complexity by extracting DNA, digesting
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