Finalized-Case Study CRISPR Cas Fall 2016 (1).docx - Cut-It-Out CRISPR case study Part I TED talk(David grandfather retired civil engineer David paused

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Cut-It-Out! CRISPR case study Part I. TED talk (David, grandfather, retired civil engineer) David paused as he walked towards his favorite reading chair, absorbing the early morning sunlight peaking through the window. Even though he was retired now, he still woke early and liked to get started on his day. Rather than sitting in an office chair working on draft plans for a new community center, he now began his day here in his living room with his iPad. He had adjusted to this change in accessing information and appreciated that one didn’t have to wait for the daily paper to see what was going on in the world. After a quick scan of the headlines - which revealed nothing too interesting - David navigated to the TED website. He had recently become hooked on TED Talks, and relied on these much more than the Discovery or History Channel to help him keep learning. In another life, he would have been a college professor… He had just finished watching the 2015 best science talks playlist yesterday (a 16 year old developing a test for pancreatic cancer? wow!), so what next? What about genetics? What was new with all that human genome research? And wasn’t his granddaughter Nadia taking a genetics class this term at UCLA? She’d be home for the holidays soon...maybe he could impress her with his up-to-date knowledge. Scrolling through his search results, a name caught his eye: Jennifer, his late wife’s name. What was this about - editing our DNA? Well, it must be fate - Jenny was a writer and editor, so this should be a good place to start his morning. Fifteen minutes later, as the closing applause signified the end of the video , David shook his head. Bacteria’s defense against viruses to curing human disease? This is why he had been a civil engineer. Living things were so much more complicated .... Questions: Worth 8 points total 1. Where did the CRISPR system first originate in nature and what is its purpose in that context? Worth 2 points 2. How is Cas9 able to bind to specific sequences of DNA? What does Cas9 do to the sequences it interacts with? Worth 2 points 3. What advantage does the CRISPR/Cas9 system offer compared to previous genome editing technologies? Worth 2 points 4. David remembers one of his friends discussing how the government should focus funding more on clinical research rather than basic research. His friend felt that basic research in model organisms was not very important anymore since we know so much about genetics. Why are models systems an important part of research? What might David tell his friend about basic research, now that he’s seen this TED video? Worth 2 points
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