wrd-ot-colorimetry-basics_445264_7.ppt - COLORIMETRY Prepared By Michigan Department of Environmental Quality Operator Training and Certification Unit

wrd-ot-colorimetry-basics_445264_7.ppt - COLORIMETRY...

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C O L O R I M E T R Y Prepared By Michigan Department of Environmental Quality Operator Training and Certification Unit Note: A printed description of colorimetry is available in the OTCU Laboratory manual (Section 310) available on the OTCU website.
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C O L O R I M E T R Y Color Measurement
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COLOR COLOR Interaction between LIGHT and MATTER
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Matter “ORBIT” Nucleus Electrons Energy Level of Electrons
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Orbits = Energy Level L e vel 1 Level 2 L e vel 3 Each Electron Can Be In Only Certain Energy Levels Each Electron Can Be In Only Certain Energy Levels
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LIGHT LIGHT Photon - “Energy Packet” wavelength - (time) frequency - (gamma) (lambda) Wave
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LIGHT LIGHT E = h hc = The Energy ( E ) of the Photon is Related to the wavelength and the frequency of the Wave   Where: h = Planck’s Constant c = Velocity of Light
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E = hc Constants Every wavelength ( ) has a specific Energy level. LIGHT LIGHT
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Summary Summary Each Each Electron Electron Can Be In Can Be In Only Certain Only Certain Energy Energy Levels Levels Every Every wavelength wavelength has a has a specific specific Energy Energy Level. Level.
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C O L O R I M E T R Y 600 nm 700 nm 650 nm
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COLOR RESULTS WHEN RADIATION IS ABSORBED RADIATION IS ABSORBED BY AN ELEMENT ELEMENT OR BY A COMPOUND COMPOUND FORMED THROUGH A REACTION W H I T E W H I T E RED BLUE YELLOW YELLOW BLUE RED ABSORBED GREEN
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TRANSMITTANCE (T) OF THE INTENSITY OF LIGHT LEAVING SOLUTION (I) TO THE INTENSITY OF LIGHT ENTERING SOLUTION (I O ) RATIO
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TRANSMITTANCE I T = I O I O I %T = T x 100
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Units of Optical Path Transmittance 0 1 2 3 4 5 1.0 .9 .8 .7 .6 .5 .4 .3 .2 .1 0 I 0 I 1 I 2 I 3 I 4 I 5 Comparing Light Transmittance to Cell Length I n
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LAMBERT’S LAW LAMBERT’S LAW Relates the absorption of light to the depth or thickness of the colored liquid Each layer of equal thickness will absorb the same fraction of light which passes through it An arithmetic increase in thickness gives a geometric decrease in light intensity transmitted
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Units of Concentration Transmittance 0 1 2 3 4 5 1.0 .9 .8 .7 .6 .5 .4 .3 .2 .1 0 I 0 I 1 I 2 I 3 I 4 I 5 Comparing Light Transmittance to Concentration I n
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BEER’S LAW BEER’S LAW Relates the absorption of light to the concentration of the absorbing substance in the solution The fraction of light absorbed is directly
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  • Fall '15
  • SARAH PARK

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