EC93859E-1886-4258-BB2F-5FE78528F7A2.jpeg - Lab 5 Detection and analysis of recombinant dihydrofolate reductase 125 Learning objectives Describe the use

EC93859E-1886-4258-BB2F-5FE78528F7A2.jpeg - Lab 5 Detection...

This preview shows page 1 out of 1 page.

Image of page 1

You've reached the end of your free preview.

Want to read the whole page?

Unformatted text preview: Lab 5: Detection and analysis of recombinant dihydrofolate reductase 125 Learning objectives Describe the use of recombinant proteins in research, pharmaceuticals, and other industries . Identify challenges and considerations for the production of recombinant proteins Suggest how expression and purification of tage design agged dihydrofolate reductase proteins could be used for further research and drug design . Analyze fusion proteins computationally through sequence analysis Analyze fusion proteins experimentally by Western Blot Identify potential sources of error/variation based on experimental procedures and analysis of your results However, before we cover relevant recombinant protein and DHFR background information... . We need to go ahead and prepare for Western Blotting today (your gels are already running and we need to be ready for setting up the transfer when they are done) . We will then have time during the transfer to review the introductory information and further consider our expected results...
View Full Document

  • Fall '07
  • Sikorski

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern

Stuck? We have tutors online 24/7 who can help you get unstuck.
A+ icon
Ask Expert Tutors You can ask You can ask ( soon) You can ask (will expire )
Answers in as fast as 15 minutes
A+ icon
Ask Expert Tutors