4mar03midterm - 7'5 MCB130 Midtenn Exam NAME K E 7 March 4...

Info icon This preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 7'5 MCB130 Midtenn Exam NAME K E 7 March 4, 2003 (page 1 cf 7 pages) Randy Scheknian, Instructnr Student LI). # TA. name (40) L Ynn have identified three different signals en the stern] regulatory element binding pretein (SHEEP). Answer the fellnwing gnestiens about these signals. a) (Q) Yen mutate the nuclear lecalizatinn signal (NLS) in the intact SREBP sn that it is tinn- fnnctinnal. Will this mntatinn cause a high level er lew level at HMG-CnA rednetase in cells that are grnwn in the absence nf LDL‘? Explain gent answer. l—fl‘“ ill-EM. Ahm’éwiw’al ”LS will melt PLFW~L 4-5,“ M,WN MMEJ 5““ l“ W“ was Mal-Wt. «an, we uh‘mm eh PMQUl‘KMi-td'hfl L43 snuggle/Lt We.“ Elam Mtg c4 ngL W'iv ufig‘ b) (E) Yen have identified the CDPflbinding signal en SREBP. and yen intrndnce- rnntatinns in this signal an that SREBP can no lnnger interact with the COPII preteins. Will this rnntatinn cause a high level at lnw level at HMG-CnA rednetase in cells that are grnwn in the absence nf LDL? Li?“ W- 1’" “Wréwclifiml awn shaman—«2W4. will km? ““3 Ell-€39 W‘WW l“ 3.4.4 (if. “Twat. 3‘” M‘W (M mi M ”kmj?arm ”ls: ALT-i 04 halal? W ll tvt'll Wk ERR (WWW c) L13) Yen identify annther signal an SREBP that is required tn "retain the pretein in the ER by virtue at an interactien with an ER ‘anehnr‘ prntein- Will rnntatinn at this signal cause a high level er lnw level nf HMG-CeA rednetase in cells grnwn in the presence of LDL? u'ra‘lAlml. m Maw Emmi gab .rxfl QREs-p is Ngumj r... H.l‘"1Atr~-49~ll SKéEP Pfiteavi‘ iv vim ' a 'm tall; mewh 41th dfiNfiml (-fii‘vfllli Madam} 1063:... [.33 L‘), A mfljl‘hilim in ass; Wall-0F 1:9»th slag will alumni-r MEEP ta lat trauma r... 4% cis going; 3%“; tat; Llama lfl-a-'- «l4: Wham; “£1ng {or fiwoclvqné JIM N-tchJ‘th-fifil %W.Tw~d’r Gaga-«x it “llN-W‘i '15 PM? 0c (L‘Uhldtlfifnl //8‘ If men 130 Midterm Exam NM KEY March 4, 2003 (page 2 ef 7 pages) Randy Sehekman, Instructer Student ID. # TA. name 11) (LU) Describe ceneiselj,r the experiments that weuld allew yen te demenstrate that the NLS in SREBP is necessaryr and sufficient fer its lecaliaatien te the nucleus. Mammy. mekakicmg in Mim‘etid Mficiw5a-€ NL; Chum wati SWEEP f “be mun ifi:N gcfiiflw‘. UH Mike“! wHHA Flumsmzlr EH" Jill {a la(1L'{-( sflfé’p w he. calls umwj ‘Md filwswq ctr" skidrmmw gal-(1 Quiéitl ‘. kmfitfl *wléruoqu NE N3 Liv: mfi'abms mm prm {es 1&9 ”afield-t J.- dflmce ”H's :vi Wallj ‘3.» 1-: [fig-2:4" W e 12% .1. W" \ an unflé em 42 rem. {23W QLS’EFELQ PTO-tint 1.11.531 chafiamjb: évem finder-«vs Formataahm ta at; (35) 2. Yen have clened a gene fer a pessible cell surface membrane pretein and find that the fwd, uj' predicted pretein lacks an N- terminal signal peptide but has twe internal hydrephebic membrane-spanning sequences separated by a leng sequence ef pel-ar amine acids. a) (_l___) Given what we learned abent the 51 gnal hypethesis draw a diagram predicting the erientatien ef this pretein after it has becerne assembled 1n the ER. Include arrews eintin te the cgteplasmic and internal glumenall surfaces ef the ER. 'l' Cm“ as . 'T 6!? 1 ensues-131 svrfatp tin-i“ l M35312? "1 b) (Q) Draw anether diagram indicating its erientatien at the cell surface {include an arrew peinting te the cell surface}. //? steam Midterm Exam NAME if EV March 4, 2003 (page 3 cf 7 pages) Randy Schekm-an, Instruetur Student ID. # TA. name c) (m) Suppose yen add another internal hydrophebic sequence by reccmhinant manipulaticn cf the gene. Draw twe pictures indicating hew this reccmhinant pretein wculd beeerne assembled in the ER and at the cell surface. d) (Q) Suppose the prutein in part g) is synthesized in a cell deficient in signal peptidase. Weuld the biesynthesis cf the pretein be affected! and if se hcw? my #62 . m was“ has; 1m N—ermw saw WW #0195 ii. ska-35%“? "(a W. 84%}! W; 491% £993; na—i’ "ad? am im Wis-M MW We; my; +N’“; how! '35 {m4 84w ucbmflm ’i’e it‘d-Hm *mgiat'al‘fm‘. e) (i) Suppcse the pretein in part g) is synthesized in a eel] deficient in signal recegniticn particle (SRP). Weuld the hiusynthesis cf this pretein be affected1 and if se, haw? \[15' Lekkixouk SKP-"¢£\L 9th WWW“ wwkfi not. Ea W e- +M S‘RP mafia we m {FMKGLflim LQAMNO ”fins-3‘ Jet“ PM“ "Wdicq be M Mi inc-RN Makofiw "Mfg 9162‘s»? 0-9 m Meagan ”am 5 w Wmfl Mala Wflk '\ MCBI3U Nfidtet‘m Exam NAIVIE k E 1 March 4, 2003 (page 4 cf 7 pages) Randy Schekman, Instructcr Student ID. # TA. name (15) 3. Yea i'sclate a mutant cell line with a deleticn cf the gene enccding the GlcNAc phcsphchydrclase, the enzyme that cleaves GlcNAc frcm lyscscmal precurscrs that have been medified by the attachment cf GlcNAc-phcsphate. Answer the fellcwin uesticns ahcut this mutant cell line. a) (:3) what is the fate cf newly—synthesized lyscscma] precurscrt preteins? M m éetNiQé “1999‘ Misfit? Wficw eti‘fimjkclrxmb; rags-5x Mme stwm- ngu-dg M 9.1mm "WW Aw mammm MM tutor-tiara? oiiissméhamh dam. h) (i) If lyscscmal precursor preteins ccntaining the nermal lccalizaticn tag are isclated frcm ncrmal (nct mutant) cells, can these prcteins bind tc the surface cf GlcNAc phcsphchydrclase mutant cells. and be internalized tn the Iyscscme cf the mutant cells? Justify: gcur answer. c) (Q) Can LDL particles bind to the mutant cells and became lccalized tc the lyscscme? J ustifl. \LU. m LTDL masher will is? Mell'olsflt ’LNO ”wing m w sushi; o6. CeImAc. MksLAcfixehu MVM C415. mats—LIBL We: Wm 3mm it» dea [~3de M 31W“ @0913. /Q/ M03130 Midterm Exam NAME REV March 4, 2003 (Page 5 ef 7 pages) Renew,»r Sehelenan. Instructer Student ID. # T. A. name (30) 4. Previde brief definitiens ef the fellewing: a) Laeteeepermease é". f9,“ flfifimg va JKM + Feelckwltww- 40$ EM—fiofl's K'm (kin-5Q 1‘91 mm H+ la 'fi \D'K‘m Mikwh —-— 1's red} Milk hatter“ fl’f ___ “all if a W-g.<k I; i' h). entlpflrt 134511952343 fl_fl§emml him ”Mal W1 “WEE“ Wm}: *WSW‘" flunk “a mwml can“ I‘M'F‘Ef, R MW WCM W (Nae 3 kW ) awake! :36 Mecfim . em 51. fifiw ..E§hh_. . :1: eat? rt erv—SEP Wk“. k 50m W e guanine flue eetlde exchanger (GEF) ”1 3 ' SF? firm A A Baum-me muse-n 6110+: - "' P... '3» ' ~ W “M ngflfi-lficé‘fl @535: Q“? fag Wm f) elefllrin 1L 'Hs CITE—“JWU m mwm 1,9. g mafiflw Wm mega flkophm ”é— mfiiffisfi hi mm; (2w M m fit: Llfizmm Saga“ [Mafiflwmamhf (113.1. pg!» :55 M42 1. 5!; Wine MShii *9ka a “mangr- H-.- ‘ Raffa- "UNL m9 at} 4iw~£ JV (mm mékw‘Hi‘n Na+d~w k3,; fife ”Mi—w degefagoflnbfi uiflgq art: ’3) WWW" PM km; [£2ng 469%? £29wa aw---«Qm mam-u am am Mam-3 “ml. m F'w‘h Sim-3?; kgfluu 1:. cKafi-LA. LW ”3* "-3.3 WEM +15; "11 W Me‘x’efiflc'm-i Skflpn a ”TN HQILYM WUA MW-i ke’g M “dim. W1! (Hugh-grab; in mm &AN&.'M. 2’22. MCB130 Midterm Exam NAME k E’ Marsh 4. 2003 (page (i af 7 pages) Randy Sehekman. Instrueter Student ID. # T. A. name (30) 5. Yeti iselate a temperature sensitive (ts) yeast mutant defective in the Ran pretein. Answer the feliewin ue'stiens abnut this mutant. a) (£1) Yen wish te eiene the gene that eneedes the Ran pretein. Using the ran ts mutant. describe the steps yell weuld take an elnne the gene TEkSt lflfi QWS W 73. Hila’f CL” w 1% ivJ‘fl'a CLQM_ {flawed ‘6“! Cam. in: "a Rush (wast QM)? fi’iflduu Jain-ks \I'E—Mafi 130% :vxira 4&5»: Elm 1‘35 516134“ N @3er kW: m 50% ‘3in ing-Jacq fir Jfl‘i H'ES'Cfi dfifi {Shh/WW 3?k)d<m3€m~lm<kg Writs!“ 'Gch ’"b (pi-arm? 1.3K Jam; W W (.ka 50w} law Mu sum alum M W 1ng imXC-sq thcg b) (i) Yeu diseever that the ts mutatien bleeks "the ability at ' Ran tn ennaert GTP te GDP. What will happen tn nueleeeyteplasmic transport when this mutant is shifted ta a high'temperature? RN €311? hmhk'jgj U LAM-filial that iW'iCNU MM. RM C3110 kWfigs WM" eccw {“ka cfikvekam {1:- mm. Jam 1 Pea-Kama saw W444 wutfltus w Watt/mi m Mao “.63th LL?” 9.3mm»? iwm “fiflkfim w'ii LSaSSeNLrQN (image-— Wm. LNKQUJ 56"“); 'PflKfl—RM AJSHM {01‘ miter-K“ twins 3.“; mass, am hi Mums mam «(Flotsam Met ‘ awn/«wt mice-46A Mmdtws wt ll LA WM... H3 MCB 130 Midterm Exam N AME k, E Y March 4, 2003 (Page 7 cf 7 pages) Randy Schekrnan, Instruct'er Student ID. # T. A. name c) (i) What will happen tn nucleecytcpiasmic transport at the permissive (lew) temperature in this mutant? ti) (1—D) Yen is'elate ancther Ran mutatien that blacks its ability to exchange nucleutide. Yen investigate the assemny and eyteplasmic transfer cf ribusemes which form frem preteins and rRNA inside the nucleus. What ferm ef Ran will accumulate in this mutant; and what will happen In rihusemes synthesized at the high temperature in this exchange mutant? ...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern