8feb02quiz - MCB 1 3r Quiz R Schelmn-m Instructcr February...

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Unformatted text preview: MCB 1 3r} Quiz R. Schelmn-m, Instructcr February 3, 2002 NAME [36% (10 pts) Ycu have purified the N33“, glucese sympcrt pennesse and wish he study,r the mechanism ef transpert by recenstitutien cf the protein in a phcsphelipid vesicle. Answer the fellewing questicns with a. werd gr phrase: a. What must yen add tn the buffer tc drive glucese uptake inte a reccnstituted vesicle? NBJF N céiucnsgk [3. Met happens tn the reaction in the presence cf vanedste'? meshing -— m: M'Pagvt a mews-irtm Art“ imfer‘t 13 EMJ‘INQj W CNN ‘kf‘e “‘5“me am e. Transpcrt is cptimum at the phesphelipid Tm. If a trenspert resctien is cendueted at a temperature below the Tm, hcw ceuld yet: adjust the cempesitien cf the phesphelipids in the vesicle tc Optimize tranEIpert at the lewer temperature? use. Packer-Lamb tows stm {skin 'bticii at" with Edi? 1;th Wflfiivé CU “MD 10404.5. //5‘...
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