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MCB130lectures5,6 - MCB130 R Schekman Membrane Transport...

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MCB130 Lectures 5, 6 R. Schekman Membrane Transport: Nucleocytoplasmic Transport I. Nuclear envelope A. Nuclear membrane 1. Pores are large structures that span junction of inner and outer membrane. 2. Transport of particles bi-directionally through pores. B. Pore complex 1. Pore complexes contain an aqueous channel through which small molecules diffuse freely and non-selectively. 2. Larger molecules are actively transported and require sorting signals (non- cleaved). 3. Transport is post-translational and does not involve unfolding and refolding of transported proteins. II. Signals and receptors A. Targeting signals 1. Different classes of nuclear localizing signals (NLS) direct import from cytoplasm to nucleoplasm. 2. Other signals mediate export (NES) from the nucleoplasm to the cytoplasm. 3. Both signals are necessary and sufficient to sustain bi-directional transport. B. Signals decoded by soluble receptors 1. Importin proteins bind cargo proteins in the cytoplasm and deliver through the pore.
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