MCB130lectures6,7 - MCB130 R Schekman Membrane Assembly...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
MCB130 Lectures 6, 7 R. Schekman Membrane Assembly: Signal Hypothesis and Translocation Machinery A. Signal hypothesis 1. Proteins destined for secretion or assembly in plasma membrane originate on ribosomes attached to endoplasmic reticulum (ER). a. Isolated complex shows nascent chains attached to ribosomes and partly embedded in ER membrane. b. Addition of components required for completion of protein chains allows proteins to be transported into ER vesicle. 2. Synthesis of secretory protein precursors in a reticulocyte lysate. a. Immunoglobulin light chain made with NH 3 + terminal 2K dalton prepeptide. b. In vitro transport coupled to synthesis when ER vesicles present during translation. c. Signal peptide cleaved during transport into ER by signal peptidase on the inner surface of the membrane. B. Cytoplasmic and membrane proteins required for translocation 1. Biochemical fractionation has revealed two components required for early events in transport.
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern