MCB130lectures9,10

MCB130lectures9,10 - MCB130 R Schekman Lectures 9 10...

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MCB130 Lectures 9, 10 R. Schekman Membrane Traffic to the Lysosome and Cholesterol Regulation A. Transport to the lysosome 1. Lysosomes in animal cells serve to turn over cell macromolecules by hydrolysis. a. Hydrolytic enzymes must be specifically transported to the lysosome where they can act. b. Lysosomal hydrolases are glycoproteins, so they must be a product of the ER. c. Precursors are transported from the ER to the Golgi body where they are sorted from proteins destined to be secreted. 2. Sorting of lysosomal and secretory proteins mediated by a specific carbohydrate determinant. a. Oligosaccharides on lysosomal glycoproteins are acted on by a Golgi enzyme that adds N-acetylglucosamine-phosphate from UDP-GlcNAc. b. The GlcNAc is then clipped off. c. The resulting phosphomonoester-modified glycoproteins are recognized by a specific receptor - the mannose-phosphate receptor that collects all suitably modified protein in the trans Golgi cisterna where the clathrin coat buds vesicles
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This note was uploaded on 04/02/2008 for the course MCB 130 taught by Professor Schekman during the Spring '08 term at Berkeley.

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MCB130lectures9,10 - MCB130 R Schekman Lectures 9 10...

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