quizAK - Quiz February 6 2008 NAME Answer but Randy...

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Unformatted text preview: Quiz, February 6, 2008 NAME Answer but} Randy Schekman, Instructor Spring MCB130 Student ID # You investigate a cell surface facilitated carrier that uses the Na+ gradient to export H+ to the cell exterior. Answer the following two questions about this carrier: 1- (19E) Is this a symport 0r antiport carrier? . mh wk Lars) '- \Ms‘n {N063 Duff'slk '=-'=> \fl 6 ‘5“ ”1 5 Dir] fi> ext-m or 2. (EELS) You reconstitute the carrier into phospholipid vesicles and trap 22Na in a buffer containing 0.15M NaCl within the vesicles during the reconstitution. Vesicles are centrifuged and resuspended in a buffer with low NaCl (0.02 M). You find that 22Na release from the vesicles is stimulated by a low pH buffer. Assuming the carrier assembles with a unique topology (not a mixture of orientations), draw a diagram of the topology of the protein and the direction of movement of Na+ and H+. visa 0L4, —-:_-:-_-=' 1Q; gum) ' W\0(M 0‘2. pug-1‘s) - .\ fieoloa‘fi \S oeeos'fif 0" +°€O\OFQ\$ '\v\ i. L- _. \I \ _ ...
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