spr02final - MCB 130 Final Questiens Spring 2002 l Ribcseme...

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Unformatted text preview: MCB 130 Final Questiens, Spring 2002 l. Ribcseme assembly begins in the nuclenlus, a specialized cempartment in the nucleus. Answer the fellcwing questiens abcut this precess. (Spts) a. Ribesemal pretein subunits have an NLS (nuclear lccaliaatien sequence). True er False? Explain yeur answer briefly. “True. fibasmall Wm mud: «first 192. info wits—w fluguus wm_ J!" ‘bEJ-fi—‘vnis’El in.ka Fiiotfin @W4-r’5u1 CL): Jr}: Jam I 5 ts b. Ribcsemes assembled in t e nucleclus have ene er mere NES (nuclear eapcrt signal). True nr False? Explain yeur answer briefly. “Tr-ea. ens “mew. whom Meshing-1L ml. mm cw M eg Jx‘e an...) m. J‘iruCErM‘keE—Q up.a~3it¢ll__ i... AM fifflfiihw_ (5 pts) c. Assembled ribcscmes diffuse back and ferth threugh the nuclear pere cemples. True er False? Explain. Fain. lashes-emu 13M. mus—h- (Niger Umch may. éflkb d£ "a J‘LG} ii)“ “WI Hatfilrswfi'l" hm" wiéuaw sumac-4M nuclear-FM. (10 pts) d. Preside a list cf the nuclear transpert factcrs required te get assembled ribcsemes into the cyteplasm. Include a diagram cf the precess and indicate hew each factnr partici- pates in the precess. Essa—Mike! J riloasam CW" 9‘ 1' [swim 3 Liwa Erica-<13 N63 Quaklelvxl; we lunar-£9 kc: 2. Membrane fusien requires SNARE preteins that bridge adjeining membranes. One theery is that SNARE preteins are necessary and sufficient te catalyze fusien as leng as ene participating membrane has a v-(vesicle) SNARE and the ether has a t-(target) SNARE. 5 ts a. Previde a brief en lanatien ef the meanin ef the twe criteria, necessa and sufficient, P g __.J_'}1 in the centext ef a pretein, such as a SNARE, which is essential fer a cell precess such as secretien. museum meets mmlfur hanger lei-wt met-ska ,6 gang: Dag/~43 ween) Pmcieq (LU -&3€‘rcu$:nh PM-Situiw‘ ng ’6 1...: 3IW~ JI , sects, was met; Mmlxfiw tin Me was We; \ovt i—Wselw..r We viii) Lax Mafia 1‘3:- LLQ’nL‘a-‘rq We Fem.» [TL-\UJL} lieu-swag {smat'u’rubfl Ni”- ? “riflhflé :mw “am? “a ‘trSNAEq mate saga—H new sci-3am, salami) qu kLaJr Wm”; Xe iigflim_ - {10 gts] b. Describe an experiment te shew that SN ARE preteins are necessary fer membrane fusien. Hui-aim “c1 SNfiRE iMUJ‘be'iM ’3an there Aria}- a Perhwl SELL? ivx ingots *1"??th biaakd rap" 41% g err-{m $41.13 3% [10 pts} c. Describe an experiment te shew that SNARE preteins are sufficient te catalyze membrane fusien. pee: mmskiwh Wes-Mu musk “EhV—SM-Afié in W ?,?u\fi'um 5C W‘Kdli '1 tngdfiflfi {HWM bueuhhm, WK JEN. Junie *bfilxu 1w” eta Quezon is? W We:ij Ur M ’ ‘t //7 ...
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