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week2_key - MCB130 week 2 solutions 1 Fill out the...

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MCB130 – week 2 solutions 1 1) Fill out the following table. Complex Found where? Active or passive? Symport or antiport? Type of molecules transported and along (with) or against gradient Proton translocating ATPase (F- Type) Inner membrane of mitochondria, Bacterial plasma membrane Active Neither H + Along/Down gradient Proton translocating ATPase (V- Type) Vacuole, Endosome Active Neither H + Against/Up gradient P-Type ATPase Plasma membrane Active Neither 3 Na 2+ Against/Up gradient 2K + Against/Up gradient Porin Bacteria, Mitochondria, Chloroplasts Passive Neither Only size- selective, molecules travel along gradient Na 2+ -glucose transporter Cell membrane (small intestine, for example) Active Symport Na 2+ Along/Down gradient Glucose Against/Up gradient Na 2+ -Ca 2+ transporter Plasma membrane Active Antiport Na 2+ Along/Down gradient Ca 2+ Against/Up gradient Facilitated carriers Plasma membrane Passive Neither Sugars, Amino Acids, Ions Along/Down gradient Histadine transport E. coli Active Neither Histadine
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MCB130 – week 2 solutions 2 Lactose permease E. coli Active Symport H + Along gradient Lactose Against gradient 2) Sphingomyelinase attacks sphingomyelin preferentially. Describe the experiment you would conduct using this enzyme to determine the difference in proportion of sphingolipids between the inner and outer surface of the lipid bilayer of a cell. A few people asked how the enzyme works. I changed the wording of the question, which originally implied that the sphingosine itself was attacked. Here’s a diagram of the synthesis of sphingomyelin. As I understand it, the enzymatic cleavage by sphingomyelinase releases the pink part of the molecule. Experiment:
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This note was uploaded on 04/02/2008 for the course MCB 130 taught by Professor Schekman during the Spring '08 term at Berkeley.

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week2_key - MCB130 week 2 solutions 1 Fill out the...

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