Worksheet4 - 1. In the following protein: S = signal...

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Unformatted text preview: 1. In the following protein: S = signal peptide, {} = hydrophobic region (a) Draw the predicted orientation of this protein in the membrane (label each segment, plus the N and C termini). (b) Draw the predicted orientation of this protein with {6} deleted. (c) Draw the predicted orientation of this protein with {4} and {6} deleted. (d) Draw the predicted orientation of the protein with the signal peptide deleted. 2. What would you expect to happen to a secreted protein if you deleted its cleavage sequence? 3. What would happen to the cell if you made a mutation that... a. Prevents Sar1 from hydrolyzing GTP? b.Disrupts the sorting signal of a secreted protein? c. Prevents Arf from binding GTP? d.Disrupts the clathrin-recognition sequence in the mannose-6-phosphate receptor? 4. Where is the V-type ATPase found, and what is its function? MCB 130 Sections 101 and 102 Week 4 - ER and Golgi GSI: Jackie Chretien jacquelineann@berkeley.edu {2} {4} {6} {8} {S} 1 5 3 7 9 5. The cisternal maturation model of the Golgi was demonstrated by labeling a 5....
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This note was uploaded on 04/02/2008 for the course MCB 130 taught by Professor Schekman during the Spring '08 term at University of California, Berkeley.

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Worksheet4 - 1. In the following protein: S = signal...

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