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Worksheet4ak - ADSW £443 MCB 130 Sections 101 and 102 Week...

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Unformatted text preview: ADSW £443 MCB 130 Sections 101 and 102 Week 4 - ER and Golgi GSl: Jackie Ch retien jacquelineann®berkeley. edu l. in the following protein: S = signal peptide, {} = hydrophobic region (a) Draw the predicted orientation of this protein in the membrane {label each segment, plus the N and C termini). (b) Draw the predicted orientation ot this protein with {6} deleted. (c) Draw the predicted orientation of this protein with {4} and {6} deleted. (d) Draw the predicted orientation of the protein with the signal peptide deieted. p 2. What would you expect to happen to a secreted protein it you deleted its cleavage sequence? 9F 7- bomld mam assoaourcd uni-HA 4&1 mm,er Mol M" fmpqlg mammal , 3. What would happen to the cell ityou made a mutation that... a. Prevents Sari trom hydrolyzing GTP? . 5M1 \NsAs-owssis 0? 6T? —-‘7 61b? “Lu—55% 4's! Man. canme “W’M‘f M Asssoda'rcs A»; £49911 cook on “15ch PWM o'Fé hum-1.15 wow-t b.Disruptsthe sorting signal oiasecreted protein? Mona-5- Lfim5€°¢+ ( “Lth hour“ a T’Mdn va\\\ EMA-tn 'wx win. 8% C. Prevents Art from binding GTP? AH: w'\\\ not «sambu one: u c,\aech¢"\n LOO-*5" magma WWW-\- Qm afigam wt,“ tun-l ms will élolgl-qu'sdoiom 4- PMamdo-sw. _ d.Disrupts the clathrin-recognition sequence in the mannose-o-phosphate receptor? £69“ 90‘“ MbPC’. Mental transpoclc m1“ \oc anon! maturaloui -— flaws mu mi kwrid/wll Fw MuPil'l 50-11305 but “"\\ 0M3 \oc. +1¥4fi3 we WPLS wed" bug'an +0 5., m 41w. “stow awnings Hey-on Uayuiomlis). 4. Where is the Vstype ATPase found, and what is its function? V~h39¢= “stavdu lfbmn) {n eldsaameohm “‘61-”: I ado-some.) M Ljsosw). (7w: w Lem’rwb new “9&5 *‘ “9‘55 M mm“) “tr—Wm Gum-Q. WM «an Ms acde-RcAhM_ Answer ices/b, MCB i30 Sections 101 and 102 Week 4 - ER and Golgi GSl: Jackie Chretieri [email protected] edu 5. The cisternal maturation model of the Golgi was demonstrated by labeling a medial-Golgi SNARE with GFP and a trans Golgi SNARE with RFP (red fluorescent protein), and then observing these proteihs in live cells. What did they see (or, what do you predict that they would see), and how does this experiment show that the maturation model is correct? (Note: it might hel to draw a schematic} gfllfi r‘ :4 M3 Emmi -llna.+ 5mm WWI/ifs Wei ugbmm Lrtcxlv‘di Ws— WM) M W D M Ungl- mAmj-Wom) we: 5mm.) whit; i‘wl mew-mu“ *dtsageeax-ccl" Uosir «M W;— mfom’is chaW-Q. Mucus), \n M ail-crawl"; nix-dbl Laolg'i we. WMMM‘Q‘ mum one“) «3mm +0 shad ammfis «A, w w. «kiwi so,“ mew-m «masts 4m. MW‘ vs W. <5. Sketch the progression of a lysosomal protein from the ER to the lysosome. Include the tollowin ( ius brief definitions for starred itemsE/ERI, Golgi, endosome*, lysosome*, GlcNAc phosphotransfel’ase’fi Mannose-éeP'h/osphate receptor*, clathrin \ acid phosphatase". ” y 9:; , ((00400 WE Calcwhcw'l‘um 6‘ f “9on K7 (‘3 racism-us \ Sosomal wok-QM Masks __ " ngV P ‘* ekaSQwasma—LJ 7 “Ma EL mg MA . Hurts . ‘3 edict-1A:- ice 4&4. manna“- Ncogn‘fiu *r lowls [GUAM U3" Fm" way: at its {slusws‘b‘mfiw‘i 9mm \o‘vHA M I {1 M d as V @edcidfc Mali calm :l‘in Ln 050m, 3.9. 1 w o -| h w", in, pH chum M 5 "J 1 % +h$vuxé 0 (“WV‘ 6am Mb?¢ / M69 _, add ekgsg-MWEL I K . wows {be ®—&icM‘- AP. " g. ._‘ Wdfimhm finm 4H”. ¢®~W WW - * cwmn'n n . . . . . _ WOSM , b L695“; finial-7W 7. in people With lecell disease, lysosomal proteins ar missmg irom t e lysosome. What is the baSis of this disease (Lei, what msu’s) enzyme is mutated), and why does this cause miss-targeting of lysosomal proteins? noli- \\\o§,¢\e.A" gm. 0.9m b5 Aim. viqu and up \Mmfutfl so?“ Laon‘l- $0 #1) \556590195, we: msch on. b.What was the experiment that demonstrated the mannose—o—phosphate receptor is not afiected by l—cell? Wins firm not-Mad («Son -r 1—9,“ adh'u‘. ewth fl mun “f. s‘mws Aime-Jr Nil??— u. 3‘9. fro’runs {mm '11 a.“ k normal 0—“51 emit-mks $393 when unf- Gho'wfi *“Aafil (av-05min; at: 51+ 0L. ...
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This note was uploaded on 04/02/2008 for the course MCB 130 taught by Professor Schekman during the Spring '08 term at Berkeley.

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Worksheet4ak - ADSW £443 MCB 130 Sections 101 and 102 Week...

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