Worksheet6 - (a cultured cells(single cells(b ±xed...

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1. Fill in this table about different types of imaging techniques (use + or - ) : GFP et al. immunofluor. EM DIC/Nomarski live imaging high resolution labeling specific molecules observing multiple structures tissue sections systems that are difficult to transform (genetically manipulate) 2. Briefly explain the difference between resolution and contrast. 3. Compare the advantages and disadvantages of immunofluorescence using direct vs. indirect labeling: 4. Give one advantage and one caveat for each of the following imaging experiments or systems:
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Unformatted text preview: (a) cultured cells (single cells) (b) ±xed tissue (e.g., formaldehyde treated) (c) double labeling experiment (multiple antibodies) (d) double labeling experiment (multiple ²uorescent tags) 5. Name three cellular processes that involve the cytoskeleton: MCB 130 Sections 101 and 102 Week 6 - Imaging and Cytoskeleton intro GSI: Jackie Chretien [email protected]
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