Worksheet7

Worksheet7 - β 4 (at 1/2 [actin]), (4) Flaments with...

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1. Draw cartoons of the three phases of actin assembly. Include kinetics arrows, indicate whether the actin is ATP, ADP+Pi, or ADP bound, and label the (+) and (−) ends. 2. What are the mechanisms of action of the actin “drugs” cytochalaisin and phalloidin, and what is their net effect on the cytoskeleton? (For example: Latrunculin sequesters monomers, leading to net disassembly of ±laments because monomers come off ±laments but cannot add on again.) 3. What is speckle analysis, and what did it demonstrate about the way actin ±laments are oriented with respect to the cell membrane? 4. Name and briefy describe the six major classes of actin binding proteins, and give one (or more) example for each: 5. What would you expect to happen to a cell with a mutation that deleted the Arp2/3 complex? 6. What are three ways that actin ±lament polymerization can be monitored in vitro ? MCB 130 Sections 101 and 102 Week 7 - The Actin Cytoskeleton GSI: Jackie Chretien [email protected]
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7. Draw actin assembly curves for: (1) actin monomers (+ATP, etc) only; (2) monomers + formin; (3) monomers + thymosin
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Unformatted text preview: β 4 (at 1/2 [actin]), (4) Flaments with gelsolin added at a speciFc timepoint. Compare nucleators: formins Arp2/3 complex mechanism (draw, label the (+) and (−) ends) # of subunits binds to Flament sides? type of structures made activators 9. What are the Fve steps of the myosin ATP cross-bridge cycle? 10. Give the function of the following proteins within the sarcomere: • CapZ • Tropomodulin • Tropomyosin • Nebulin • Troponins (T, I and C) • Titan 11.The critical concentration of an actin Flament at steady state is 0.2uM; the Cc for the plus end is 0.1uM and the Cc for the minus end is 0.8uM. If you assemble actin to steady state in the presence of a plus-end capping protein, what will be the concentration of free actin in your reaction ? MCB 130 Sections 101 and 102 Week 7 - The Actin Cytoskeleton GSI: Jackie Chretien [email protected] time flament assembly add gelsolin 8....
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This note was uploaded on 04/02/2008 for the course MCB 130 taught by Professor Schekman during the Spring '08 term at Berkeley.

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Worksheet7 - β 4 (at 1/2 [actin]), (4) Flaments with...

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