This preview shows pages 1–2. Sign up to view the full content.
This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: β 4 (at 1/2 [actin]), (4) Flaments with gelsolin added at a speciFc timepoint. Compare nucleators: formins Arp2/3 complex mechanism (draw, label the (+) and (−) ends) # of subunits binds to Flament sides? type of structures made activators 9. What are the Fve steps of the myosin ATP cross-bridge cycle? 10. Give the function of the following proteins within the sarcomere: • CapZ • Tropomodulin • Tropomyosin • Nebulin • Troponins (T, I and C) • Titan 11.The critical concentration of an actin Flament at steady state is 0.2uM; the Cc for the plus end is 0.1uM and the Cc for the minus end is 0.8uM. If you assemble actin to steady state in the presence of a plus-end capping protein, what will be the concentration of free actin in your reaction ? MCB 130 Sections 101 and 102 Week 7 - The Actin Cytoskeleton GSI: Jackie Chretien [email protected] time flament assembly add gelsolin 8....
View Full Document
This note was uploaded on 04/02/2008 for the course MCB 130 taught by Professor Schekman during the Spring '08 term at Berkeley.
- Spring '08