102 QUIZ 2 - MCB102 Quiz#2 9/20 Please write your name on...

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MCB102 Quiz#2 9/20 Please write your name on the back of the quiz. You may use pencil or pen. Question 1 (8 points) 1a. Draw the peptide with the sequence EDR at pH 7. pKa of terminal amino group is 9.7 pKa of terminal carboxyl group is 2.2 pKa of E’s side chain is 4.2 pKa of D’s side chain is 3.6 pKa of R’s side chain is 12.5 1b. What is the net charge of the peptide at pH 7? Show your work. 1c. What is the net charge of the peptide at pH 3? Show your work. 1d. What is the net charge of the peptide at pH 11? Show your work. 1e. In which pH range does the isoelectric point (pI) of this peptide occur? A. below pH 3 B. between pH 3 and pH 7 C. between pH 7 and pH 11 D. above pH 11
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MCB102 Quiz#2 9/20 Question 2 (7 points) Chromatography is a widely used technique for separating proteins based on their different properties. Four proteins are described below: Myoglobin: Molecular weight = 16,700 Da Uncharged Lysozyme: Molecular weight = 14,400 Da Very positively charged Pepsin: Molecular weight = 34,000 Da Very negatively charged Urease:
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