Lab report 5 - Microbiology.docx - Name Angelica Castillo Student Number C18467106 Programme DT212 Date 5th December 2018 Title Physical and biochemical

Lab report 5 - Microbiology.docx - Name Angelica Castillo...

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Name: Angelica Castillo Programme: DT212 Student Number: C18467106 Date: 5 th December 2018 Title: Physical and biochemical characterisation of bacterial isolates Aim/s: To describe the colonial morphology of bacterial isolates using macroscopic and microscopic methods, and to perform a gram stain and biochemical tests (3% KOH test, catalase test and oxidase test). Materials and Methods: As detailed in handbook. Results: Colonial Morphology Description Escherichia coli ( E. coli ) Streptococcus pyogenes (S. pyogenes) Form Circular Circular Elevation Raised Raised Margin Entire Entire Surface Mucoid, smooth, glistening Mucoid, smooth, glistening Consistency Butyrous Butyrous Colour White White-gray Gram Stain Bacteria Escherichia coli ( E. coli ) Streptococcus pyogenes (S. pyogenes) Description Pink, gram-negative, rod- shaped Purple, gram-positive, chain of spheres Biochemical Tests Escherichia coli ( E. coli ) KOH Positive (+) Catalase Positive (+) Oxidase Negative (-) Streptococcus pyogenes (S. pyogenes) 3% KOH Negative (-) Catalase Negative (-) Oxidase
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