LDH paper

LDH paper - Agric B iol Chem 45(1 305-306 1981 305 Note Existence of Lactate Dehydrogenase Isozymes in Potato Tuber Tissue MasamiMuto Tadao

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Agric. B iol Chem ., 45 (1), 305-306, 1981 305 Note Existence of Lactate Dehydrogenase Isozymes in Potato Tuber Tissue MasamiMuto, Tadao Hasegawa and Takao Suzuki Department of Agricultural Chemistry, TokyoUniversity of Agriculture, Setagaya-ku, Tokyo 156, Japan Received January 7, 1980 The lactate dehydrogenase [E.C. 1 .1 .1 .27] (LDH) iso zymesin animals havebeen widely investigated in recent years. However,few studies of plant LDH have been conducted, and their isozyme characteristics are not well- known.1>2) In this instance, the distribution of LDHwas studied first in rice plants, corn seeds, dasheen tubers, sweet potato roots, potato tubers, beet roots, and squash rinds and pulp. Amongthem, potato tuber tissue showed the strongest activity (Table I). Subsequent purification and characterization of the potato enzymeclearly demon- strated the existence of an isozyme. Assim ilar findings had not been reported previously, isozyme in potato tuber tissues is herein described. The LDH activity was assayed by the method of Wroblewski and LaDuewith the slight modification of using a0.05 mphosphate buffer (pH7.0).3>4) Theactivity wasdetermined to be the catalytic reaction required in a 1m l solution to convert 1 jumol of NADH into NADin one min at 34°C and pH7.0. Protein...
View Full Document

This note was uploaded on 04/02/2008 for the course CHEM 153l taught by Professor Kim during the Spring '08 term at UCLA.

Page1 / 2

LDH paper - Agric B iol Chem 45(1 305-306 1981 305 Note Existence of Lactate Dehydrogenase Isozymes in Potato Tuber Tissue MasamiMuto Tadao

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online