LDH paper - Agric Biol Chem 45(1 305-306 1981 305 Note...

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Agric. Biol Chem., 45 (1), 305-306, 1981 305 Note Existence of Lactate Dehydrogenase Isozymes in Potato Tuber Tissue Masami Muto, Tadao Hasegawa and Takao Suzuki Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156, Japan Received January 7, 1980 The lactate dehydrogenase [E.C. 1.1.1.27] (LDH) iso zymes in animals have been widely investigated in recent years. However, few studies of plant LDH have been conducted, and their isozyme characteristics are not well- known.1>2) In this instance, the distribution of LDHwas studied first in rice plants, corn seeds, dasheen tubers, sweet potato roots, potato tubers, beet roots, and squash rinds and pulp. Amongthem, potato tuber tissue showed the strongest activity (Table I). Subsequent purification and characterization of the potato enzyme clearly demon- strated the existence of an isozyme. As similar findings had not been reported previously, isozyme in potato tuber tissues is herein described. The LDH activity was assayed by the method of Wroblewski and LaDue with the slight modification of using a 0.05 m phosphate buffer (pH 7.0).3>4) The activity was determined to be the catalytic reaction required in a 1ml solution to convert 1 jumol of NADHinto NADin one min at 34°C and pH 7.0. Protein
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