bis103win07finalkey

bis103win07finalkey - BIS 103-002 FINAL Winter 2007 K E—...

Info iconThis preview shows pages 1–15. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 10
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 12
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 14
Background image of page 15
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: BIS 103-002 FINAL Winter 2007 K E— '1 Instructor: Callis Name March 17,2007 LAST NAME FIRST NAME Student ID # If it a lies to ou, lease check the a roriate box below Ready for srin 1/ Comletin Incomlete - m Note: There are 7 questions and a total of 15 numbered pages counting the cover sheet. A blank sheet is included in the back for scratch paper and to cover your exam. Do not use this paper for your answers. If you need more room use the back of the numbered pages. Partial credit will be given. WRITE YOUR NAME ON THE TOP OF EACH PAGE. Question Value Score T= 298° K R: 8.315 J x mol‘1 x K'1 Faraday's constant (F) is 96.51:] x volt“1 x mol '1 AGO' for the reaction ADP + Pi —> ATP + H20 = +305 k] x mole ‘1 AGO' = — n F AEO' AG = AGO' + RT ln [products/reactants] for on + 1 e--—> Ared E = EO' - E In Ared nF on de novo , meaning from anew, meaning synthesis of a compound from non-related, typically simpler common metabolites. 1, _____..____, authorize the University to distribute publicly this graded exam (e.g., handed out in class, or left in a bin for me to pick up). Signature Date BISlO3-002 FINAL Winter 2007 K E 2 Instructor: Callis Name 1 March 17,2007 LAST NAME FIRST NAME 1 (16 pts) a. (10 pts). True or False. On the line in front of each statement, write T (for True) if the statement is true, or F (for False) if the statement is false. I Aminotransferase reactions are characterized by the transfer of an a-amino group from an amino acid to the a—keto position of an or —keto acid. l The term glucogenic amino acid refers to amino acids that are degraded to yield succinyl CoA, pyruvate, a-ketoglutarate, fumarate and/or oxaloacetate. T Allostery is typically the most rapid modulator of enzyme specific activity. T Nearly all known free-living organisms use similar, if not the same, metabolic pathways in catabolizing energy—containing molecules, giving rise to the concept of metabolic unity among organisms. F Covalent modification of enzyme activity is defined by the covalent addition of a phosphoryl group from ATP. T A major function of NADPH is to provide reducing power for anabolic pathways. I Uncouplers reduce the proton gradient across a membrane allowing unregulated electron flow. F The stoichiometry of photons required per NADPH produced in the light reactions of photosynthesis is eight. F Many enzymes of the Calvin-Benson—Basham cycle (C3 cycle) are similar to those of the TCA cycle and reductive pentose phosphate pathway. L Tetrahydrofolate is required for regeneration of the methylating co—factor, S—adenosyl methionine. BIS 103-002 FINAL Winter 2007 3 Instructor: Callis Name March 17, 2007 LAST NAME FIRST NAME lb. (6 pts). Because you (humans) are unable to biosynthesize de novo several biomolecules that are essential for cellular function, you must obtain them, or their immediate precursors, from your food to stay healthy. For each type of molecule listed below, name 91155 specific compound of that type 9; a direct precursor for a compound of that type that you have to eat. If there is more than one possible, just write one. Fattyacid “NoLilC LWDMNK AC‘D Amino acid 10F 7H2 N771“. ALA, Electron carrier cofactor N N " " lei 50PM V IN methyl carbon carrier cofactor FD MC AL 1 W M g T Reduced sulfur H i T ° ' («33.12110 5 Reduced nitrogen in humans 318103-002 FINAL Winter 2007 key 4 Instructor: Calm Name March 17, 2007 LAST NAME FIRST NAME 2. (10 pts). Corn meal is high in tryptophan and low in lysine. In contrast, protein from lima bean seeds is low in tryptophan and high in lysine. (a). Which is more nutritious- a meal of 1000 calories of succotash (a mixture of com and lima beans) OR a meal of 1000 calories of corn? Briefly explain why in terms of biochemistry discussed in class. ®AMMWM§5_ . EMA {W Sfiutfia *i k ’5 . - . (’9 by» . 5M fliwwfib Mama; mam 00 £0.11 EDD-«MT Ania/m “W; WA» mum. “"“h , " i w ‘ »wv i clay m MAM “‘"afigyw Mew *2 “W20 3 was (b). Which is more beneficial for human nutrition? A single meal of 1000 calories of succotash every day, or a single meal of 1000 calories of corn meal one day, followed by a single meal of 1000 calories of lima beans the next day? Briefly explain your answer in biochemical terms. :3: We? in Mm 0ime » M90300, :0 if 05W 6 W 9W mm, m a (ensign 3 $313 we 0% “f; 1‘ at 04mg. (“maki— Witth {L QWMAE a a, w M @34er W 000» Mm mfis, / BISIO3-002 FINAL Winter 2007 i a 5 Instructor: Callis Name March 17,2007 LAST NAME FIRST NAME 3. (6 pts). a. (4 pts). An “Activated” intermediate is a key component in an anabolic pathway whose subsequent conversion allows the synthesis of a more complex molecule to be energetically possible. For each of the biomolecules listed below to the left that are end products of a biosynthetic pathway, write the name or draw the structure above the line to the right of the “activated” intermediate required for its biosynthesis. Glycogen ‘ 6 “Activated intermediate” Fatty acids MA LON‘GL C D A “Activated intermediate” PQPP “Activated intermediate” Purine and pyrimidine nucleotides $3- av O~ Mir}; L, SiK “Activated intermediate” de novo cysteine biosynthesis (b). (2 pts). Describe the most interesting concept/fact you learned in this class. BISl03-002 FINAL Winter 2007 E \1 Instructor: Callis Name L March 17, 2007 LAST NAME FIRST NAME 4. (14 pts). The biological carbon cycle can be simplistically summarized as follows: Organic carbon compound Catabolism Anabolism (:02 (a). (3 pts) Name all the pathway or pathways and specific reactions (if not in a pathway) that accomplishes the catabolism of organic carbon compounds completely to C02. [Just the carbon pathways» WWW: “5 PDH arm coo—E Ctp+ 1m) w ‘OX'DATION TC (5 (1,115) a (If-‘4- OV AA - BmLDLJ-aSI: -9 TfiA (b). (1 pts) Name the pathway or pathways that accomplishes the anabolism of C02 to organic carbon compounds. CALVIN (“301.5 (c). (5 pts)v What single enzyme—mediated reaction accomplishes the initial assimilation of C02 in the anabolic portion? Write the reaction and name the enzyme. Write a balanced reaction. Eli BISCO (1:39 Kmtosufimm + 007, + H10 -—-» ax 309A (we) ('12) (M (42) ('11) 318103-002 FINAL Winter 2007 L1 7 Instructor: Cant Name March 17, 2007 LAST NAME FIRST NAME ((1). (5 pts). The processes of mitochondrial election transport, bacterial electron transport, and the light reactions of photosynthesis are strikingly similar: they both are electron flows coupled to the synthesis of ATP. Which gum component is functionally analogous to the following mitochondrial components? Name the chloroplast component. 1. ubiquinone (£oenzyme Q)? +) 1. pugs"? avuv/N‘a M 2 P3) 2. cytochromec? 4" 2. 3. Complex 111? +| 3. W5 59/! 4. matiix? ’i’ ‘ 4. Lumw’ 5. intermembrane space? +\ 5. 318103-002 FINAL Winter 2007 E2 01 8 Instructor: Callk Name ‘ March 17, 2007 LAST NAME FIRST NAME 5. (10 pts) For the following enzymes, DRAW the structures of the substrate(s) and product(s) for the most likely reaction. Nag, but do not draw the structures of nucleotides and eo-factors. You may use Pi for inorganic phosphate. Write balanced reactions. Indicate with arrowhead(s) the physiological direction(s) of each reaction. lull a homoscrine aminotransferase L/j‘g‘b 0V5.“ w ‘3‘ fig v n 6'“; g“; (.HLBPLP €in 3y ~ . 4 cu; ’ .. I m 5 ‘3’ 1 ._ "" (Mi—(5' [IX ‘9 (Th: "’r Cm H‘C'th‘D 9 ‘fl ' L a 1 4‘ l + c, 0 l 3 m m C 0 0 "‘ (T9 5 b. Ribose—S-P ketolisomerase +33 +\» 5 («GOD I H r__ 0 H o H a 2 ‘ 1 - m) as wind C. aspamite decarboxylase _ o " (4' ’72, \ // 0 D b ‘S‘f’ 3 L702. I (ILH Z L ‘ l H - (>401: ‘ 9 W C H; I’VH * ' +72- 0:} 3 ' H ‘ 3 BISl03-002 FINAL Winter 2007 9 Instructor: Callls Name E; 1 March 17,2007 LAST NAME FIRST NAME 6. (10 pts). A hypothetical branched biochemical pathway is shown below. The capital letters indicate metabolic intermediates (metabolites) whose inter-conversions are catalyzed by enzymes. The names of the enzymes are written above, or to the left of the reaction arrows (for example, NZ#2 catalyzes the inter-conversion of compounds B and C). The directionality of the arrowheads indicates physiological directions of the conversions. The questions below refer to this diagram. NZ#l NZ#2 NZ#3 NZ#4 NZ#S l E NM ll F (a.) (5 pts). On the line below, write the name of all the enzymes of this branched pathway likely to be remade!) {H} in H l 14 4+ H 5“ __._._l___________t_______.__ Justify your choices. Why did you pick these? (:27 Pfl‘wu‘M‘fl’” W‘ mm. (b.) (3 pts). Of the enzyme names you wrote in (a), name one enzyme that is likely to be the most highly , regulated. («ti j is I Why did you pick this one? i. (32} 164 54¢}, (W; pdtflmnva/ Sm WM *5: “WK? vrfi'wm m,» (c) (2 pts). If metabolite F was an allosteric regulator of this pathway, predict whether it would function as an activator or inhibitor. Circle one. wwwcmylmno-Wu- .l ( INHIBITOR ) or ACTIVATOR BIS 103-002 FINAL Winter 2007 10 Instructor: Callis Name 1 March 17, 2007 LAST NAME FIRST NAME 7. (34 pts). Multiple choice (2 pts. each). Circle the best answer. There is only 1 correct answer per question. a. Which of the following statements is true about oxaloacetate (0AA) in humans? i. It can be formed by the carboxylation of pyruvate in the cytoplasm. ii. It can be formed by the oxidation of malate. iii. It can be reacted with acetyl CoA and water to form citrate in the matrix of the mitochondria. iv. It can be formed from aspartate following an aminotransferase reaction with glutamate. ® All of the above. vi. None of the above. b. The standard free energy change for the hydrolysis of ATP is —30.5 kJ/mol. Measurements in rat liver cells indicate that the concentrations for both ATP and ADP are 8 mM and for inorganic phosphate is 1 mM. Calculate the free energy change for this hydrolysis reaction under cellular conditions at 37°C (298°K). i. -59.6 kJ/mol -47.6 kJ/mol iii. —l3.4 kJ/mol iv. +13.4 kJ/mol v. -27 kJ/mol 0. Which of the following statements is false (not true) about the coenzyme tetrahydrofolate (T HP)? i. It is derived from the NADPH—dependent reduction of biopterin. ii. Bacteria cannot perform the de novo synthesis of tetrahydrofolate (in other words, cannot use a non—THF—related compound, such as glucose and ammonia, as biosynthetic precursors). iii. It can carry carboxyl groups at the N5 position. iv. Tetrahydrofolate is only required for amino acid biosynthesis and plays no role in nucleotide synthesis. ® All of the above are false. vi. None of the above are false. BIS 103-002 FINAL Winter 2007 S £1 11 Instructor: Callis Name March 17,2007 LAST NAME FIRST NAME d. Which of the following statements about nucleotides is false (not true): i. They are produced by nearly all organisms. ii. The rate of cell division can determine the amount of nucleotides required per unit time. iii. Uridine nucleotide derivatives of carbohydrates are common intermediates in synthesis of oligo— and polysaccharides. iv. Many coenzymes are derivatives of nucleotides. 6') Dietary purines and pyrimidines are an important energy sources in humans. 6. After several days of starvation or fasting, the human brain: i. Converts triacylglycerides that it has stored in its own cells into ketone bodies for use as an energy source. Utilizes beta—hydroxybutyrate that is circulating in the blood. iii. Utilizes its glycogen stores to provide glucose for energy. iv. Utilizes its stored protein to provide glucose for energy v. Does all of the above. vi. Does none of the above. f. Fatty acids are released from triacylglycerides stored in fat cells (adipose cells) when: i. insulin levels are high. ii. glycerol-3—P levels are high in adipose cells. blood glucose levels are low. iv. adipose ATP levels are high. v. All of the above are incorrect. vi. None of the above are incorrect. BISIO3-002 FINAL Winter 2007 Kw Instructor: Callis Name March 17,2007 LAST NAME FIRST NAME g. Uses of glucose—6—phosphate (indirect and direct) in mammalian liver are: i. Conversion to pyruvate for alanine biosynthesis ii. Synthesis of NADPH. iii. Synthesis of ribose—S—P iv. Hydrolysis to glucose for release into the blood stream v. Synthesis of glycogen ® All of the above are uses for glucose-6-phosphate. vii. None of the above are uses for glucose—6—phosphate. h. Ethanol metabolism in the human liver has the following properties: i. Ethanol is not catabolized by the liver, because all is either excreted or breathed out. i. ii. Ethanol catabolism results in its oxidation to acetate and acetate is excreted. Ethanol catabolism alters the NAD/NADH ratio that was previously balanced by glycolysis and the electron transport chain. iv. Ethanol synthesis occurs under anaerobic conditions in liver cells. v. None of the above. I In plants and microorganisms, synthesis of all 20 protein amino acids is typically a matter of synthesizing the appropriate , followed by an aminotransferase reaction using i. acetyl CoA derivative; glutamate a—keto acid; glutamate iii. reduced N NH3; glutamine iv. reduced N NH3; glutamate v. a-keto acid; glutamine 12 BISlO3-002 FINAL Winter 2007 E i 13 Instructor: Callis Name March 17, 2007 LAST NAME FIRST NAME j. uses seven of the same enzymes as glycolysis, but must replace three enzymes of glycolysis because these reactions are i. TCA cycle; physiologically irreversible. iii. Catabolism; reductive. Gluconeogenesis; physiologically irreversible. iv. Gluconeogenesis; located in a different subcellular compartment. v. Pentose phosphate pathway; located in a different subcellular compartment. k. The final products of aerobic carbohydrate catabolism are: i. pyruvate and H20 ii. lactate iii. ammonia and H20 ® co2 and H20 v. NADH and acetyl CoA l. The free energy change (AG) for any reaction at equilibrium is: i. greater than 1. ii. equal to - RT In Keq. iii. can be utilized to do work in the cell. 2610 . v. less than 1. BIS 103-002 FINAL Winter 2007 K 14 Instructor: Callis Name March 17, 2007 LAST NAME FIRST NAME m. When the amount of carbohydrate obtained from the diet is greater than the energy needs of the body, some of the excess carbohydrate is converted to and some is converted to , both for storage purposes. i. amino acids; glucose ii. protein; glycogen iii. glycogen; ketone bodies iv. triacylglycerides; protein G glycogen; triacylglycerides n. Which of the following is (are) characteristics of anabolic pathways? i. assembly of complex molecules ii. formation of new covalent bonds iii. ATP provides energy iv. NADPH is an electron donor ® all are characteristic of anabolic pathways vi. none are characteristic of anabolic pathways 0. The function of glycogen phosphorylase is: i. the conversion of glucose-l-phosphate to glucose—6—phosphate ii. to break down ATP to catalyze the phosphorolysis of glycogen iv. to catalyze glycogen synthesis v. to inhibit the production of glucose-l-phosphate. BIS 103-002 FINAL Winter 2007 K i ‘1 15 Instructor: Callis Name March 17, 2007 LAST NAME FIRST NAME p. Fructose in the diet or fructose from sucrose in the diet can be a source of energy for fatty acid synthesis in the liver because: i. ketoses are fattening ® fructose enters glycolysis through phosphorylation iii. fructose provides a net of four ATP from glycolysis iv. the glycerol backbone of triacylglycerides comes specifically from fructose. v. none of the above. q. The enzyme catalyzes the reaction: fructose + —> + ADP i. phosphofructokinase-l; ATP; fructose-6-P. ii. phosphofructokinase-I; ATP; fructose-1,6-bisP iii. hexokinase; Pi; fructose-6-P iv. 'fructokinase; Pi; fructose-6-P Q fi'uctokinase; ATP; fructose- 1 -P ...
View Full Document

This note was uploaded on 06/17/2009 for the course BIS 103 taught by Professor Abel during the Spring '08 term at UC Davis.

Page1 / 15

bis103win07finalkey - BIS 103-002 FINAL Winter 2007 K E—...

This preview shows document pages 1 - 15. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online