MidtermOneKey2009

MidtermOneKey2009 - M IDTERM 0N2 BISlO3-001 -— FIRST...

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Unformatted text preview: M IDTERM 0N2 BISlO3-001 -— FIRST MIDTERM 2 April 28, 2009 Name 1. (24 pts). The words below refer to either a structure or a reaction. Match the word with a correct structure or reaction shown below. More than one letter may be correct, but put only ONE letter in the space in front of the word. A letter may be used more than once and some may not be used, but only one answer per line. The structures and reactions continue on the NEXT PAGE. E) I 3"3’ Phosphorylation reaction '1 Phosphorylase Phosphatase reaction 1 V) Phosphorolysis reaction F Phosphofructokinase B Phosphate ester I H or Dehydration reaction MSW 6 of K Hydrolase G L NW4. D R Dehydrogenase High Energy compound Hexose ‘ B Glycolytic Intermediate A. CH0 B. - c. D. 2 0P03' H H /0 HC HC/O HO H HO H H H H H H H H ‘ H 2- CHZOH C 20303 CW0” CHQOH E' F- 0“on CH20P032' opof‘ O -O\C¢O \ngo H0 H HO H H—-'—OH fi H+OH ; CH20P032' ATP ADP CH20P032' CHQOH CHon ' CHQOH CH20P032' H20 HPO43' CHgOH CH20P032- H20 CH20P032‘ 313103—001 —— FIRST MIDTERM K E 5 April 28, 2009 Name 2. (12 pts). For the following enzymes, draw the structures of the substrate(s) and product(s) for the reaction they catalyze. Write balanced reactions. Include the names (no structures) of any co-factors, nucleotides if required for the reaction. You may use Pi for inorganic phosphate if required for the reaction. Indicate with arrowheads the physiological direction e reactions. 9'" +HC03’MTP ‘9 éfo +AD+0‘,' CH3 ® an; I Coo- @oiuate lyase QQVQML is C“ an)" _ IMST ONE l L _ CH3C00- 4» 901 HO‘ C wow .._.—5 Jr 0:0 ' ‘ I 942 @ wz C” it i in " ® -6-P ketolisomerase BISIO3-001 — FIRST MIDTERM K g April 28, 2009 Name 2 3. 20 pts. 1 3a. (11 pts). Fill in the blanks. Z w: (a CL. If a person takes in more glucose than the body needs at that time, the body stores the extra glucose in the cells of the l I V (/V and lMfig If; - (names of organs) in a form called ’ N . The immediate biosynthetic precursor is 2. (put a number) ATP (s) (or ATP equivalent)/per glucose. The major user of blood glucose in the body is the B N (name of an organ). In between meals, when blood glucose begins to fall, the (name of organ) releases glucose into the blood. The enzyme that catalyzes the first step is called 0’1”) V (name ofenzyme). V Humans lack the ability to synthesize ethanol, but can cataboliZe ethanol. The first step in the metabolism of ethanol by the liver is its oxidation to (name of compound) in a reaction catalyzed by (name of enzyme). This reaction requires the co—factor (name of cofactor). BIS103-001 -.FIRST MIDTERM April 28, 2009 Name 3b. (4 pts). Using structures, draw the balanced reaction catabolizing ethanol in humans: Lug-CH1!) H + NAD‘ r-z‘ U43 0:: + NAM-l + H1" c. (5 pts). The catabolism of ethanol in the liver affects the conversion of pyruvate into lactate in the liver. Would you predict that the presence of ethanol would increase or decrease the rate of synthesis of pyruvate into lactate? or DECREASE (CIRCLE ONE) xii; For full credit, you must explain your answer. {5 Wk + NADH++ H* —-> WATi may 50 we H mat/«fr; Réaumis NADH, WHICH lemmaqu Pm Arm—l. “0&5 NADH mow. 315103-001 -- FIRST MIDTERM April 28, 2009 Name 8 Last, First (print clearly, please) 4. (14 pts). In glycolysis, fructose-1,6-bisphosphate (F-1,6bisP) is converted into glyceraldehyde-3-P (G-3-P) and dihydroxyacetone phosphate (DHAP). a. (3 pts). Draw the structures of the reactants and products and name any co-enzymes (if required for the reaction) for this reaction. L p I (’20 _ Mo 0 ® 0 H e > C‘MLOH \ ~0H H640 ‘ "g afflbsb H +014 + p b. (5 pts . e AGO' for this reaction is + 23.9 kJ mol-l. Calculate the equilibrium constant (Keq) at 37°C. For full credit, you must show your work and place your answer on the line °’ A6 we" + em» C? 6v a+ u}; A1710. 0 3 Ab" +IQT'KKLJb 2': .. 1 3571:71‘;ii‘¢} ' 318103-001 -- FIRST MIDTERM April 28, 2009 Name 9 Last, First (print clearly, please) Is this reaction SPONTANEOUS o NON-SPONTANEOUS Ey'rcle one) un r standard conditions? d. (5 pts). The following concentrations of reactants and products were measured in mammalian red blood cells: F1,6-bis P 31 x 10'6 M DHAP 140 x 10'6 M G-3-P 19 x 10‘6 M What is the AG in mammalian red blood cells? (assume T= 37° C). For full credit, you must show your work and place your answer on the line below. @9159 :2. A901 than“ Q a ,, ‘5 (“23,4003”), 4- («my M) M “‘0’” “X m” W“ V “3! Mb" (a e a?) z 25m a + (2 57%) 9,; fuzz/0‘5] “Wm” 1: 13,000 + (153;) ("7.30) = 13,900 _ 24/39 AG= " O. 7-9" K3 BISlO3-001 -— FIRST MIDTERM April 28, 2009 Name 10 Last, First (print clearly, please) 5. (8 pts) Short Answer . a). (4 pts). You are in charge of a research team that is trying to create a new bacterium from chemically synthesized DNA. This bacterium will derive all of its ATP from sunlight by photosynthesis. Members of your team decide not to include genes encoding enzymes of the TCA cycle. Do you agree or disagree with this decision? For full credit, please briefly explain why you agree or disagree. AGREE or (circle one) TcA Mace, WW4: Saw, mumfiiafl bWS‘thtVZ pmmsors, TW'UWW b). (2 pts). What is the advantage of synthesizing glycogen with a high frequency of branches? c). (2 pts). A patient at a medical clinic has muscle fatigue after exercising. Analysis of his glycogen revealed a higher level of muscle glycogen than normal with short branch lengths. What activity is most likely to be deficient in this patient? Name the enzyme. Diewcwt N-Z. Enzyme 315103-001 -- FIRST MIDTERM April 28, 2009 Name ‘ 11 Last, First (print clearly, please) 6. (22 pts). Multiple Choice. Circle the best answer. There is only one best answer per question. Each question is worth 2 pts. m l Z ! . ’ (4 5 4 M Ma. Lim’ffi 3 p“ i. The complete aerobic oxidation of 1 mole of glucose generates a net of __ moles of ATP. a 20 b 24 c. 30 CE 38 e. 40 ii. How many moles of ATP from the complete aerobic oxidation of 1 mole of glucose’are derived from substrate level phosphorylation? a. 2 b. 3 CE; 4 d. 20 e. 24 iii. Acetyl CoA labeled with 140 in both of its acetyl carbons (both the carbonyl and methyl group) and unlabeled oxaloacetate (0AA) are incubated with a crude liver extract capable of carrying out the reactions of the TCA cycle. After one turn of the TCA cycle, the 0AA present in the extract was isolated and the presence of 14C at each position was determined. This pool of 0AA would have label in: a. none of its carbon atoms b. the two carboxyl carbon atoms § all four carbon atoms . the keto carbon and one of the carboxyl carbons e. the pattern cannot be predicted from the information provided in the question B18103-001 -- FIRST MIDTERM April 28, 2009 Name 12 Last, First (print clearly, please) iv. The of pyruvate to acetyl CoA is catalyzed by a. dehydration, pyruvate dehydrogenase b. transacylation, pyruvate dehydrogenase c. carboxylation, pyruvate carboxylase oxidative decarboxylation, pyruvate dehydrogenase e. oxidative decarboxylation, pyruvate carboxylase v. The reaction of malate oxidation to oxaloacetate is not thermodynamically favored under standard conditions. This conversion does occur in cells because: it involves substrate level phosphorylation it is coupled to a strong reduction reaction it is coupled to 'ATP hydrolysis oxaloacetate is used in the next reaction which has a negative .AG. 969m oxaloacetate is used in the next reaction which has a negative AGO’. vi- Ilamidaiivgeéegarbaszlalas 99: seams reactions in which but-using eareiraqnired.' _ required? a. Lipoic acid b. NAD+ c. Thiamine pyrophosphate d. CoenzymeA I Q) Biotin 315103-001 -- FIRST MIDTERM April 28, 2009 Name 13 Last, First (print clearly, please) vii. The first energy rich compound produced in glycolysis is: @ 1,3 bis-phosphoglycerate b. 2-phosphoglycerate c. glyceraldehyde-B-P d. phospho-enol pyruvate e. Fructose-1,6bisP viii. The isomerization of glucose-6-P to fructose-G-P provides a hexose able to be cleaved into trioses by aldol cleavage b. synthesizes an aldose sugar from a ketose sugar c. is a physiological irreversible reaction d. is an ATP-requiring step e. requires NAD+ ma aw ix. The term “physiological irreversible” for a reaction means: a. the reaction can never proceed in the direction written. b. the reaction does not require an enzyme for conversion under physiological conditions. Q the reaction proceeds only in the direction written under physiological conditions. d. the reaction has a large negative standard free energy change. e. the reaction has a large positive standard free energy change. nm%#s x. Lactate, rather th 11 pyruvate, is a product of anaerobic metabolism in the bacterial species Lactobacillus acidophilus because a. lactate has fewer carbons than pyruvate b. lactate can be exported from cells, while pyruvate cannot @ NADH oxidation is required for continued ATP production by glycolysis d. these organisms do not require any TCA cycle reactions 9. none of the above are good reasons ...
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This note was uploaded on 06/17/2009 for the course BIS 103 taught by Professor Abel during the Spring '08 term at UC Davis.

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MidtermOneKey2009 - M IDTERM 0N2 BISlO3-001 -— FIRST...

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