midterm2win02blankandkey

midterm2win02blankandkey - Winter 2002 BIS103-002 MIDTERM 2...

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Winter 2002 BIS103-002 MIDTERM 2 February 26, 2002 Name_______________________________ page 1 Student ID # _______________________________________ Check the appropriate boxes below Undergraduate Graduate Audit Completing Incomplete Note: There are 6 questions and a total of 9 numbered pages counting the cover sheet. A blank sheet is included in the back for scratch paper and to cover your exam. Do not use this paper for your answers. If you need more room use the back of the numbered pages. Partial credit will be given. WRITE YOUR NAME ON THE TOP OF EACH PAGE. Question Value Score 1 22 2 20 3 15 4 20 5 16 6 7 TOTAL 100 T= 298º K R= 8.315 J x mol -1 x K -1 Faraday's constant (F) is 96.5kJ x volt -1 x mol -1 Δ G o ' for the reaction ADP + P i ATP + H 2 O = + 30.5 kJ x mole -1 Δ G o ' = - n F Δ E o ' Δ G = Δ G o ' + RT ln [products/reactants] I, ______________________________, authorize the University to distribute publicly this graded exam ( e.g. , handed out in class, or left in a bin for me to pick up). Signature ________________________________________ Date _____________________
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Winter 2002 BIS103-002 MIDTERM 2 February 26, 2002 Name_______________________________ page 2 1. (22 pts). (a). (8 pts). In controlled and properly monitored experiments, one group of turkeys is fed 14 C- succinate and another group is fed 14 C-palmitate (a C-16 long chain fatty acid). After 24 hours, the livers of both turkeys were found to contain 14 C-glycogen. When diets of succinate and palmitate were continued over a long period, the succinate-fed turkeys thrived (grew larger in size and were healthy), whereas the palmitate-fed turkeys did not survive (ie., died). During this experiment, both groups of turkeys obtained no other source of carbon except sufficient vitamins, salts, and just enough amino acids to synthesize the proteins that they needed. Qualitatively explain both these two results in terms of the metabolism of these compounds; that both groups contained radiolabeled glycogen in their livers after 24 hours, but only the group of turkeys fed succinate survived. Mention specific biochemical pathways. (b). (4 pts). Would the answer be different if a C-17 long chain fatty acid were fed to the turkeys instead of a C-16 fatty acid? Explain why or why not. Please mention specific biochemical similarities or differences in your answer.
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Winter 2002 BIS103-002 MIDTERM 2 February 26, 2002 Name_______________________________ page 3 (c). (6 pts). In a different controlled and properly monitored experiment, one sample of yeast is fed 14 C- succinate and another sample is fed 14 C-palmitate (a C-16 long chain fatty acid). These compounds serve as the only source of carbon for these organisms. After 4 hours, the glycogen content of both yeast samples were analyzed for the presence of 14 C-glycogen. Long-term growth studies were also performed. Would the results be the same for yeast as for turkeys (results for turkeys are described in part a above)? Why or
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midterm2win02blankandkey - Winter 2002 BIS103-002 MIDTERM 2...

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