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FinalExamSpring09KEY

FinalExamSpring09KEY - 13151034101 FINAL Spring 09...

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Unformatted text preview: 13151034101 FINAL Spring 09 Instructor: Callis Name June 10, 2009 LAST NAME, FIRST NAME Please print clearly ' Student ID # BIS 103-001 FINAL Spring 09 L E E! 4 Instructor: Callis Name June 10,2009 LAST NAME, FIRST NAME Please print clearly 1. (10 pts.) Fill in the blanks. (a). (4 pts.) Glucose-6-P is a intermediate in or the initial starting compound in multiple pathways in the human liver. Write the names of 4 of these pathways on the lines below. 1. Gwamsw 2_ GLMCONCbéENESK (0N5 3. Guam em S‘ONTHiSl s 4, QéNmsciHflanIerE Maw/MUM) 1b. (6 pts). Complete the following sentences. Write the correct words in the spaces provided below the sentence. The sentences are not related to each other. I. Two major types of enzyme regulatory mechanisms that modulate enzyme specific activity discussed in class with multiple examples are a and b a. 4% ‘ Covalml’ mo mime cuHW‘ #1de b. diode/Q II. If methionine is not present in the diet of humans, the amino acid c (name a specific amino acid) can substitute for methionine, serving as a precursor for synthesis of new methionine. c. HOMUCwi‘Ull/m _' III. Plants synthesize methionine from the carbon compound d (name the amino acid biosynthetic family) and the source of sulfur is e I d_ 0AA (OXALOACETAT9! e. Cage/NZ $03“ 6' Qflsfth-Houmz IV. f provides the reducing power for anabolic processes. f. NADPH . BIS 103-001 FINAL Spring 09 LEE ( 5 Instructor: Callis Name June 10, 2009 LAST NAME, , FIRST NAME Please print clearly 2. 18 pts. a. (10 pts). The overall reaction shown below is the sum of three different enzymatic reactions. Write out the three separate reactions below in the space provided, using names of compounds or structures. Name co—factors if required, but do not draw the structures of co—factors. t1??? win/VD z, W iMIIW" 5‘2; Hi. US 2 Glycine + NAD+ it mHgO Pyruvate + C02 + 2 NH3 + NADH Reaction 1: We) : - 0 . ® @ @ NADH +140 Reaction 2: g 0:"). .5 ® 3, 6L, we +11%anst ms: *Hbo -——A 3R +THF .\ ~ \ 5 Reaction 3/: .__ ® 6 52a M5“ WK + M43 2b. (4 pts). What single enzyme catalyzed reaction is directly responsible for most of the initial incorporation of NH3 into an organic compound in the cells of plants, bacteria and fungi? (Write the name of the enzyme and write out the reaction). @ 6le S‘owTHir/tée NHg’réLa MW ——3 éLA/ +A00+P @®@ 2c. (4 pts). What single enzyme catalyzed reaction is directly responsible for most of the synthesis of glutamate in the cells of plants, bacteria and fungi? (Name the enzyme and write the reaction). @ Ik/fiwa gum «2pr 0I( ,a QLU’I’AMAT‘E scan/THAéE. BIS]03-001 FINAL Spring 09 K E )6 6 Instructor: Callis Name“ June 10,2009 LAST NAME, FIRST NAME Please print clearly 3. (12 pts). Matching (a) ( 6 pts). Subcellular Localization. The metabolic pathways and enzyme reactions that we have studied this quarter are not all localized in the same subcellular compartment in eukaryotes. For the pathways or enzymes listed below, indicate their subcellular location. If a pathway occurs in more than one location, indicate all subcellular compartments. Each compartment may also be used more than ince. Do not guess. nucleus cytosol lysosome mitochondrial matrix inner mitochondrial membrane microbody (=peroxisome, glyoxysome) chloroplast thylakoid chloroplast lumen chloroplast inner membrane chloroplast outer membrane chloroplast stroma @ /_ K‘l'vi’k wenmpwwpcw> NR...“ > i. 5 animal glycogen synthesis beta oxidation of fatty acids in animals ~__D_ TCA cycle 8 substrate level phosphorylation 0“ 1" ID D( 6.2%} oxidative phosphorylation PPP (pentose phosphate pathway) 6 anaerobic glycolysis K Calvin cycle F?) fatty acid synthesis in animals H water oxidation in PS G" Plastoquinone reduced (=PQH2) —> Plastoquinone oxidized (=PQ) + 2H+ + 2e' H (:5 “J Plastocyanin (red) —> Plastocyanin (ox) B18103-001 FINAL Spring 09 K E\ / Instructor: Callis Name_ June 10,2009 LAST NAME, FIRST NAME Please print clearly (b). (6 pts-0.5 pts each). The next page contains structures of compounds we have encountered this quarter. Place the letter of the compound in the space provide next to its corresponding name. Only 1 answer per line. Some compounds may be used more than once, some not at all. _8__ a carotenoid _D_ Pyridoxal phosphate (PLP) L NAD“ ___C__ a chlorophyll L FAD i ATP i Thiamine pyrophosphate (TPP) __H_ glucose i Coenzyme A (CoA) _E_ biotin _E_ __':_ Tetrahydrofolate (THF) S-adenosyl: methionine (SAM) BISlO3-001 FINAL Spring 09 K E Y . i 9 Instructor: Callis Name June 10, 2009 LAST NAME, FIRST NAME Please print clearly 4 (16 pts). For the following enzymes, draw the structures of the substrate(s) and product(s) for the most likely reaction catalyzed by the enzyme. Do not draw the structures of nucleotides or co-factors. You may use Pi for morganic phosphate. Write balanced reactions. Write the name of any cofactors required for the reaction on the line provided. If no cofactor is required, write “none” on the line. a. aspartate aminotransferase . Carp” [K A" C01) I @ H '7} (lb; ' > o R ‘13 ’ i l ' i - + C an , J Cf ~ c:0 3» ~ m h} ”H3 — '1 — + ”L 9‘) ' + I (EH2 C "7. l l I ‘ . - ,C i o - ,C= o . W fH‘ O 0 1C: d r“ o 9 Cofactor(s) 0L? Li 5flw ,5 b. G-3-P ketolisomerase lrk (.7 O eHLOH .. ~ (“1.5 H Jl‘ o H H C : a “/7 ‘ \ir' . 1 ., ( Q l 5 13,-; U ,\ a My C“; (in I . ,C:a 1 Z d. RuBISCO N /\ ‘ i" i {w w 0 \ '1 r \L/ t 1* ‘ :9 0:0 N M l \‘x \S’; 10-‘0H f Cal J‘ “to "a 4” lc‘OH (3.5 (1’0“ \N/l ~ ‘ ' C or ‘ \‘5 Cofactori'sgl ‘0 p” 2" H 313103-001 FINAL Spring 09 5'; 10 Instructor: Callis Name é‘\ i,» .7 June 10, 2009 LAST NAME, FIRST NAME Please print clearly 5. (9 pts.) Short answer. (a). (3 pts). Vegetarians that do not drink milk, eat eggs or any other animal product are especially advised to eat food from different species of plants at the same meal, for example to combine corn-containing foods (corn tortillas or corn muffins, for example) with legumes, such as re—fried beans or peas. What is the biochemical basis for this dietary recommendation? l—lwmams amt ,W‘ (AU 30 (ii/Diem ammo (AW/LS HUM scralClrLColc new), EDI/iii??? lib-rite aroma W M‘sl' in. ob 1’0 ("(4 m Hw Mt In 6146(9qu “MED/Wis (Wesfln‘ilal ammo 0‘“de EAAS) 80m plam‘l’ Food have lnSMfFiélenl' amomvfs 0’1) m wot {w EAAs and 0W Marni—Based 'FbOds have insufficient amom‘l’s cl) diFéci/ont EMS when ta‘l'cn +ng-Au‘1-Ac’ztc l5 . . Sufiiuovd'amm'ts immmf. (b). (3 pts). Your pet gerbil was mistakenly given a diet of starch as the only source of food in his diet. After several weeks, you notice that the leg muscles of your gerbil are smaller than they were several weeks ago. What is the biochemical basis for the loss of muscle mass in your gerbil during this starch only diet? Mammda MW! (Mill/J Mi’ala, 3)) mm M,‘ steaks lemma/N5 elm WSJL iv!) A HM 50% (MW WWO WCMl’ Q—w‘fl’ t’AAe. for “‘6‘ - gcgnmsis 5], pva-u‘ns 4» MokMSaéssmfiafl «car sun/ml (c). (3 pts). The energetics of photophosphorylation and oxidation phosphorylation are both based on the same fundamental mechanism. What is that mechanism? Give it a name and briefly describe it in the space provided. Pi P/wlaww Mom 53mm (50% Wliac LL ch'avaabifivi’l’ l-fl PWVldQfll—Q 6‘“ W 6” ATP Sammy‘s. ‘ 313103-001 FINAL Spring 09 K E y p 1 1 Instructor: Callis Name“ June 10, 2009 LAST NAME, FIRST NAME Please print clearly 6. (30 pts). Multiple choice (2 pts. each). Circle the best answer. There is only 1 correct answer per question. i. The bacterial enzyme glutamine synthetase is highly regulated by multiple allosteric regulators. The following compounds are likely to be allosteric regulators of bacterial glutamine synthetase EXCEPT: a. glutamine glutamate Q malate adenine e. aspartate ii. All of the following statements about nucleotides are true EXCEPT: —/a. They are synthesized de novo by nearly all organisms. b. The rate of cell division can determine the amount of nucleotide synthesis required per unit time. c. Purines and pyrimidines are synthesized using mostly amino acids as the source of nitrogen atoms. d. Several co-factors are derivatives of one or more nucleotides .ietary purines and pyrimidines are important energy sources of energy in humans. iii. An enzyme with the name acetate kinase most likely catalyzes the following: a. acetate + ATP +CoA ~> acetyl coA + ADP +Pi .acetate + ATP —> acetyl-phosphate +ADP c. acetyl-phosphate —> acetate +Pi d. acetyl CoA +ADP —> acetate + ATP + CoA e. acetate + UTP —> acetyl-UMP + pyrophosphate BIS 103-001 FINAL Spring 09 12 Instructor: Callis Name June 10, 2009 LAST NAME, FIRST NAME Please print clearly iv. All of the following are characteristics of phenylketonuria (PKU) EXCEPT: a. PKU results from a deficiency in phenylalanine hydroxylase activity. ® In PKU patients, phenylalanine incorporation into protein is reduced. c. PKU is treated by putting patients on a diet that is low in phenylalanine. d. Untreated patients suffer severe mental retardation. e. Symptoms of PKU also occur when one or more enzyme required for catalyzing biopterin synthesis are defective. v. The synthesis of amino acids is different between plants and humans for several reasons. One aspect that is different is ' a. Humans use several amino acids as precursors for purine and pyrimidine synthesis whereas plants do not. ® Plants biosynthesis all 20 protein amino acids from scratch, whereas humans cannot synthesize all protein amino acids from scratch. c. Humans synthesize amino acids using NH4+ from nitrate reduction, whereas plants do not. (1. Humans use cysteine as a source of reduced sulfur for synthesis‘of methionine, whereas plants use the sulfur from methionine in the synthesis of cysteine. e. none of the above. vi. A name for the type of enzyme that catalyzes a NAD+~dependent oxidation of 3-PGA to hydroxy— phospho—pyruvate is: a. isomerase b; epimerase c. hydrolase ® dehydrogenase e. kinase 313103—001 FINAL Spring 09 Instructor: Callis Name June 10,2009 ‘ LAST NAME, FIRST NAME Please print clearly vii. The AGO’ values for the three reactions shown below are given. Malate + NAD+ —> oxaloacetate + NADH + H+ AG°’ = +29.7 kJ/mol malate dehydrogenase Oxaloacetate + acetyl-CoA + H20 —> citrate + CoASH AGP’ = -32.2 kJ/mol citrate synthase Oxaloacetate + acetate ——> citrate AGO’ = -1.92 kJ/mol citrate lyase What is the AGO’ for the hydrolysis of acetyl-CoA? Acetyl-CoA + H20 -> acetate + CoASH a. -2.5 kJ/mol @ -30.3 kJ/mol c. +61.9 kJ/mol (1. +303 kJ/mol e. -61.9 kJ/mol viii. The following is (are) properties of co—enzymes: a. They participate in the catalytic reaction. b. They can become covalently linked to one or more substrate. 0. They may be synthesized from vitamins. (1, They may be covalently linked to the enzyme. 6. They may be modified as a result of enzyme activity. f . None of the above All of the above 13 BIS 103-001 FINAL Spring 09 14 Instructor: Callis Name June 10, 2009 LAST NAME, FIRST NAME ix. xi. xii. Please print clearly Three common regulators of the enzymes of glycolysis and TCA cycle typically are: .ATP, NAD+, ADP b. ATP, thiamin, NAD+ c. adenine, thiamin, riboflavin d. none of these lists are correct e. all of these lists are correct Which list of amino acids contains amino acids most closely interrelated biosynthetically? aspartate, methionine and glutamate. alanine, glycine and serine. a. b. @ asparagine, aspartate and methionine. d. alanine, aspartate and methionine. e. glycine, serine and glutamate. All are true for CAMP-dependent protein kinase EXCEPT: a. I activated by increased levels of adrenaline in the blood. b. phosphorylase kinase is a substrate 6:) cAMP is a substrate (1. phosphorylates glycogen synthase e is activated in response to increased blood glucagon The first intermediate of glycolysis with Gibbs free energy of hydrolysis more negative than that of ATP is: a. Phosphoenol pyruvate b. fructose-1,6-phosphate c. GTP d. 3-phosphoglycerate 1,3 bisphosphoglycerate BISlO3-001 FINAL Spring 09 K Ey Instructor: Callis Name June 10,2009 LAST NAME, FIRST NAME Please print clearly xiii. Biological oxidation reduction reactions that we have studied this quarter always involved: transfer of hydrogens formation of water mitochondria transfer of electrons .6... direct participation of oxygen xiv. Which of the following statements about the light reactions in higher plant photosynthesis is false? There are two distinct photosystems, linked together by an electron transfer chain. ATP synthase couples ATP synthesis to electron transfer. The ultimate source of electrons for the process is H20. a b c. The ultimate electron acceptor is 02. e Ferredoxin is an electron carrier in this system. xv. Starting with Fructose-1,6bisP, the conversion of Fructose-1 ,6bisP to two moles of pyruvate by the glycolytic pathway results in the net formation of: a. one mole of NAD+ and two moles of ATP b. one mole of NADH and one mole of ATP c. two moles of NADH and two moles of ATP .. two moles of NADH and four moles of ATP e. two moles of NAD+ and four moles of ATP xvi. Which of the following is true of feedback regulation? 1. Feedback regulation modulates the last step of a biosynthetic reaction. 2. Feedback regulation prevents futile cycling. 3. - Feedback regulation only operates in catabolic reactions. @Feedback regulation helps control flux into biochemical pathways. . Feedback regulation covalently modifies an enzyme to control flux. 15 B18103-001 FINAL Spring 09 K E X Instructor: Callis' Name June 10, 2009 LAST NAME, FIRST NAME Please print clearly 7. Short opinion answers. (a). ( 2 pts). The most interesting piece of information or concept I learned this quarter was: ANTS thw new «all. Mafia. (b). (1 pt). A piece of advice I would give future BIS 103 would be: 16 ...
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