Review_Exam - Study Guide for Exam 2[Lectures 11-19 MMG 301...

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Study Guide for Exam 2 [Lectures 11-19] – MMG 301 Summer 2007 Nutrition/cultivation [Lecture 11] Vocabulary : macronutrients, micronutrients, cofactors, autotroph, heterotroph, phototroph, lithotroph, organotroph, chemoheterotroph, chemolithotroph, photoheterotroph, photoautotroph, fastidious, growth factor, growth factor analog, de novo synthesis, Concepts : what are the functions of vitamins in the cell; how are para-amino benzoic acid and niacin utilized know differences between a defined and undefined growth medium; what is agar used for differences between selective and differential culture media; how can production of gas by a culture in a tube containing a liquid growth medium be demonstrated advantages and disadvantages of autoclaving and filtration methods for sterilization what is aseptic technique; review the 4 methods of isolating pure cultures; which methods can have subsurface colonies review Most Probable Number method; what is this method used for; how is it calculated briefly review the colony morphology terms what is an enrichment culture; what is an example of its use Cell Division/Diff/Measurement & Batch/ Continuous Culture [Lectures 12 & 13] Vocabulary : Durham tube, agar, sterilization, autoclave, pure culture, septum, endospore, propagule, sporulation, spore acid- soluble proteins, dipicolinic acid, Petroff-Hausser counting chamber, laser tweezers, turbidity, generation time, exponential growth, semi-log plot, growth rate constant, batch culture, inoculum, Liebig’s law of the minimum, washout, signal molecules, Concepts : what is MRSA; what did a recent study find about the prevalence of MRSA in US hospitals review the Bacillus, Paenibacillus , and Clostridium species discussed in class; Paenibacillus papilliae, Bacillus thuringiensis, B. anthracis, Clostridium perfringens, C. tetani, C. botulinum review the types of cell division found in bacteria what are the key events in spore formation; in what microbe has this process been best studied review the 4 most common methods for measurement of bacterial populations; advantages and
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