Lieb_Prob_Set_9_Key

Lieb_Prob_Set_9_Key - Biology 202-Lieb Problem Set 9...

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Biology 202-Lieb Problem Set 9 4/18/07 1. The DNA sequence of the coding strand for a gene is shown below. The number on each line corresponds to the position of the first nucleotide on that line. PCR is performed using the primers 5'-GCCCGCCTTCCAGATAAAC-3' and 5'-GCGGAAAATGCCGCCAAA -3'. What is the expected size of the product? 375 bp 1 ATTTAATTAT GGCCGATTCG T GCGCGGAAA ATGCCGCCAA A GGGACGGAA 51 AATGAGCGGC CGAAGAAGTG GAGGCCAGCG CCGACACCGT CCCACAGGTG 101 GAGGAGGGCG TAGAGGAGTA TTTGAAGCGT AAGAACATGG TCCTCCTCGA 151 CTACGAAAAG CAAATGTTTC TCGATTTGGT CGAGGCCGAT GGTCTGCTGG 201 TTTGCGCCAA GGGCCTGAGC TACGACCGCG TTGTAATCAG CATCCTCAAG 251 GCCTACAGCG ATTCCGGCAA TCTTGTGCTT GTGATCAACA GTTCTGACTG 301 GGAGGAGCAG TATTACAAGT CCAAAATCGA ACCCAAATAT GTCCACGAAG 351 GTGCCAGCAC GGCCACCGAA AGAGAGCGC G TTTATCTGGA AGGCGGGC TG 401 CAGTTCATTA GCACGCGCAT CCTGGTGGTG GATCTGCTCA AGCAGCGAAT 2. A 60 bp DNA sequence is written in double-stranded form below (the dots mark every 10 th base pair). . . . . . . 5'-TTACCCGATCCTGAAATCCATACTTAATGTCCCGCTTCAATTCCGCGTAGACATCGTGCC-3' 3'-AATGGGCTAGGACTTTAGGTATGAATTACAGGGCGAAGTTAAGGCGCACCTGTAGCACGG-5' a) Which if the following pairs of primers could be used in a PCR reaction with this DNA sequence as a template (only one is possible)? A
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Lieb_Prob_Set_9_Key - Biology 202-Lieb Problem Set 9...

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