Molecular Biology of Sickle CellIn sickle cell disease, there is a mutation in the gene that encodes the chain of hemoglobin. Within this gene (located on chromosome 11), one basein the DNA is replaced with another base, and this mutation causes the normal amino acid #6 to be replaced by another amino acid.Making a Normal Beta Chain of HemoglobinThe sequence below is the first part of the long DNA sequence for theβ chain of normal hemoglobin. Fill in the complementary DNA strand.Making Sickle Cell HemoglobinIn sickle cell disease, there is a mutation at the 17thnucleotide of DNA in this gene. Fill in the complementary DNA strand.DNA Strand 1CACGTGGACTGAGGACTCCTC...DNA Strand 1CACGTGGACTGAGGACACCTC...Transcription: Now make the messenger RNA from the new complementary strand of DNA (Strand #2) that you just wrote down. Use the RNA base pairing rules.mRNAGUGCACCUGACUCCUGAGGAG...mRNAGUGCACCUGACUCCUGUGGAG...Translation: Now, using a codon chart, translate this mRNA into a sequence of amino acids. You may use the single letter abbreviation for each amino acid.
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