Study_Guide_for_Lab_Competency_ExamF06Fi - Study Guide for...

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Study Guide for Lab Competency Exam Lab Competency Exam (LCE) Dec 4 -7 (during your scheduled lab section) 50 pt A. Every student should have signed up for LCE. You signed up for a specific time and need to attend at that time. Showing up late for your time or at a different time may result in an automatic 0. B. Your lab instructor can add to this study guide as much as he or she wants. C. Students missing the LCE should call Sonya to determine if and when they can take the make up. Call her immediately at 432-1316 ext 138. D. The time for each station is 8 minutes. E. There is no extra time at the end; so make sure you look over your exam when you get done with a station early. F. There are 5 stations in the room which everyone must complete. G. Once you leave a station you cannot go back, so if you needed a letter for an answer you cannot look back. By the end of the semester, you should also be able to: 1. Perform all basic techniques (measuring, balancing, streaking etc.) learned in this lab and subsequent labs. 2. Identify each tool, safety item and piece of equipment used in this lab. 3. When given data for an experiment, determine whether the data is accurate or not based on how the technique is performed (e.g., isolation streaking technique did not yield isolated colonies but only had a lawn of growth-someone did not perform the correct technique or the student did not sterilize the loop between zones; the centrifuge is not balanced; spectrophotometer data that had not been adjusted (zeroed) with the blank so you cannot create a standard curve, etc.) 4. Describe what is the purpose for using the following different pieces of equipment: centrifuge, microscope, spectrophotometer and gel electrophoresis box. 5. Complete the following tasks for these 5 stations . Spectrophotometry, Biochemical Testing and Dilutions 1. Be able to zero the spectrophotometer with the blank and explain why the spectrophotometer is "zeroed." 2. When given different samples mixed with a biochemical dye, be able to put each sample into a spectrophotometer and record the absorbance of each sample at a specified wavelength. 3. When given various tubes with samples, be able to determine which order the tubes and cuvettes should be used in the spectrophotometer. 4. When given samples, be able to collect the data for a known protein sample. Be able to properly use a spectrophotometer, zero the spectrophotometer and determine the absorbance of each diluted sample. 5. Be able to set up a dilution manually or by illustrating it or calculate how to perform a dilution. Be able to solve a dilution problem (C i V i = C f V f ) or create a diluted solution by using the various tools and materials provided. 6.
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Study_Guide_for_Lab_Competency_ExamF06Fi - Study Guide for...

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